Profil Dr.Totik Sri Mariani

Dr. Totik Sri Mariani

School of Life Sciences & Technology
Institut Teknologi Bandung
Phone: +62 22 251 1575, 250 0258
Fax: +62 22 253 4107
Email:totik@sith.itb.ac.id

Education
  • S1 Biologi FMIPA-ITB, 1990
  • S2 Agricultural Science, Nagoya University, 1993
  • S3 Bioagricultural Science, Nagoya University, 1999
Research Interest
  • 1. 2007–2008 Induction of natural oil production in Jatropha curcas L. in vitro. Plant Sciences and Biotechnology Research Group. Institut Teknologi Bandung . (member).
  • 2. 2006–2008 In Depth Study for Developing Efficient Direct Somatic Embryogenesis of Jatropha curcas : Morphology and Ultrastructure . Funded by Asahi Glass Foundation , Japan . (Principal Investigator).
  • 3. 2005-2007 Application of somatic embryogenesis and microspore embryogenesis technology in providing oil palm synthetic seeds for field trial. Funded United Prime Research (RUT) MENRISTEK. (Principal Investigator).
  • 4. 2000–2004 Effect of 2,4-D on indirect somatic embryogenesis and surface structural changes of somatic embryo in garlic (Allium sativum L.) cv. Lumbu Hijau, Laboratory of Plant Physiology, Department of Biology, Institut Teknologi Bandung, Bandung, Indonesia. (Principal Investigator).
  • 5. 1999-2003 Morphological Study on Direct Somatic Embryogenesis of Lithospermum erythrorhizon and Its Shikonin by Scanning Electron Microscopy. Funded by Asahi Glass Foundation , Japan . (Principal Investigator).
  • 6. 1995-1999 Studies on direct somatic embryogenesis in rice (Oryza sativa). Funded by Monbusho , Japan . (Principal Investigator).
Karya Terpenting
  • 1. Epidermal Cell Wall Biogenesis with Emphasis on Cuticular Layer Formation during Direct Somatic Embryogenesis in Rice. 1999
  • 2. Improvement of Direct Somatic Embryogenesis in Rice by Selecting the Optimal Developmental Age of Explant and Applying Desiccation Treatment. 2000
  • 3. Direct somatic embryogenesis in Rice (Oryza sativa L.) : Structural and developmental patterns. 2002
  • Effect of 2,4-D on indirect somatic embryogenesis and surface structural changes in garlic (Allium sativum L.) cv. Lumbu Hijau. 2003
  • 4. Pengaruh metil jasmonat pada induksi dihydroechinofuran selama embriogenesis somatik Lithospermum erythrorhizon Siebb.etJucc. 2004.
Publications
  • 1. Mariani, T.S., Zuirda, M and Miyake, H.  2012 . In depth study for developing efficient direct somatic embryogenesis of Jatropha curcas L. : Morphology and Ultrastructure. International Journal of Basic and Applied  Sciences. Vol. 12 No. 06:20-28
  • 2. Mariani, T.S., 2011 . Morphogenetic aspects of somatic embryogenesis in monocotyl and dicotyl plants. Biosainstifika. Vol 2. No.2 : 192-207
  • 3. Mariani, T.S., Fitriani, A., Wicaksono, A., and Chia, T.F.  2011 . NMU-induced mutation in Aglaonema by particle bombardment. International Journal of Basic and Applied Sciences Vol: 11 No 03:59-67
  • 4. Mariani,T.S. , Fitriani, A., Teixeira da Silva, J.A., Wicaksono, A., and Chia, T.F.  2011 . Micropropagation of Aglaonema using axillary shoot explants. International Journal of Basic and Applied Sciences Vol:11 No.1:  46-53
  • 5. Mariani, T.S ., Wardjo, N.G., Miyake, H. 2006. Initiation and Proliferation of Globular Somatic Embryos of Oil Palm (Elaeis guineensis, Jacq.). Planta. Submitted.
  • 6. Mariani, T S, Latif, Sjafrul, Ginting, G2 Indrianto, A, and Miyake, H. Somatic embryogenesis of oil palm (Elaeis guineensis Jacq.) for synthetic seed production; 2006; International Oil Palm Conference. Bali.
  • 7. Mariani, T.S ., Ramayanti, O., Yazaki, K. and H. Miyake. 2004. Development of Somatic Embryo in Lithospermum erythrorhizon Siebb, et Zucc. And the Study on the Effect of Methyl Jasmonate on Its Maturation. Annales Bogorienses. 9(2):72-79.
  • 8. Mariani, T.S. Ramayanti, O., Pengaruh Metil Jasmonat pada Induksi Dihydroechinofuran Selama Embriogenesis Somatik Lithospermum erythrorhizon Siebb, et Zucc; 2004; Seminar MIPA IV ITB.
  • 9. Mariani T.S ., Miyake H., R.R. Esyanti and I. Nurwendah. 2003. Effect of 2,4-D on indirect somatic embryogenesis and surface structural changes in garlic (Allium sativum L.) cv. Lumbu Hijau. Jurnal Matematika dan Sains ITB 8(4) : 133-139. Mariani T S. Miyake H. and Takeoka Y. Direct somatic embryogenesis in Rice (Oryza sativa L.) : Structural and developmental patterns; 2002; Jurnal Matematika dan Sains ITB
  • 10. Mariani T S, Yazaki K. and Miyake H. Morphological Study on Somatic Embryogenesis of Lithospermum erythrorhizon by Scanning Electron Microscopy; 2002; 10th International Association of Plant Tissue Culture and Biotechnology Congress. Orlando, USA.
  • 11. Mariani, T.S., Nurwendah, I., Esyanti, R.R., and Miyake, H.,. Changes in surface structure during indirect somatic embryogenesis in garlic (Allium sativum L.) cv. Lumbu Hijau observed by scanning electron microscopy; 2003; International Seminar on Biotechnology for Sustainable Agriculture: State of the art of Research and Product Commercialization, SEAMEO BIOTROP, Bogor.
  • 12. Ramayanti, O., Mariani, T.S. and Miyake, H. Somatic embryogenesis of Lithospermum erythrorhizon Siebb, et Zucc; 2003; International Seminar on Biotechnology for Sustainable Agriculture: State of the art of Research and Product Commercialization, SEAMEO BIOTROP, Bogor.
Publikasi Lain:
  • 3.Lithospermum
Presented Papers
  • Mariani, T.S . and Erlangga, B.P. 2006. SEM Study on Early Stages of Oil Palm (Elaeis guineensis Jacq.) Somatic Embryos. International Conference On Mathematics and Natural Sciences. ITB, Bandung .
  • Mariani, T.S ., Latif, S., Ginting G., Miyake, H. 2006. Somatic embryogenesis of oil palm (Elaeis guineensis Jacq.) for synthetic seed production. International Oil Palm Conference. Nusa Dua, Bali .
  • Mariani, T.S Ramayanti, O.. 2004. Pengaruh Metil Jasmonat pada Induksi Dihydroechinofuran Selama Embriogenesis Somatik Lithospermum erythrorhizon Siebb, et Zucc. Seminar MIPA IV ITB, Bandung .
  • Ramayanti, O.,  Mariani, T.S. and Miyake, H. Somatic embryogenesis of Lithospermum erythrorhizon Siebb, et Zucc. International Seminar on Biotechnology for Sustainable Agriculture: State of the art of Research and Product Commercialization, SEAMEO BIOTROP, Bogor . 2003
  • Mariani, T.S. , Nurwendah, I. , Esyanti, R.R., and Miyake, H.,. Changes in surface structure during indirect somatic embryogenesis in garlic (Allium sativum L.) cv. Lumbu Hijau observed by scanning electron microscopy. International Seminar on Biotechnology for Sustainable Agriculture: State of the art of Research and Product Commercialization, SEAMEO BIOTROP, Bogor . 2003
  • Mariani T S , Yazaki K. and Miyake H. Morphological Study on Somatic Embryogenesis of Lithospermum erythrorhizon by Scanning Electron Microscopy. 10th International Association of Plant Tissue Culture and Biotechnology Congress. Orlando , USA . 2002.
  • Mariani T S . Miyake H. and Takeoka Y. Improvement of Direct Somatic Embryogenesis in Rice by Selecting the Optimal Developmental Stage of Expl and Applying Desiccation Treatment. Meeting of the International Program on Conference of Rice Biotechnology, Phuket , Thailand . 1999.
  • Mariani T S. Miyake H. and Takeoka Y. Epidermal Cell Wall Biogenesis with Emphasis on Cuticular Layer Formation during Direct Somatic Embryogenesis in Rice. Conference of Japanese Crop Science, Kyoto , Japan . 1998.
  • Mariani T.S., Miyake H. and Takeoka Y. Changes in surface structure during direct somatic embryogenesis in rice scutellum observed by scanning electron microscopy. Conference of Japanese Crop Science, Kochi , Japan . 1997.
With my background of knowledge and experiences, I am interested in doing research especially on plant tissue culture. Our community require innovative and applied research development in order to solve many problems around us. Curently, our team are conducting several topic of researches and development such as :
  • Micropropagation of leather leaf fern.
  • Micropropagation of orchids.
  • Somatic embryogenesis and secondary metabolite production in Lithospermum erythrorhizon.
  • Somatic embryogenesis of garlic.
  • Somatic embryogenesis of oil palm.
  • Synthetic seeds production of oil  palm .
RESEARCH & CONSULTANCY EXPERIENCES
Year Topics Position Funding Institution
2006-2007 In depth study for developing effecient Direct Somatic Embryogenesis Of Jatropha Curcas : Morphology and Ultrasctructure PI Asahi Glass Foundation
2005-now Application of somatic embryogenesis and microspore embryogenesis of oil pallm in providing synthetic seeds production and its trial in field PI Minitsry of Research and Technology, Indonesia
2004-now Micropropagation of leather leaf fern PI PT Algabon
2004-now Micropropagation of orchid PI PT Algabon
2003 Somatic embryogenesis of garlic PI Asahi Glass Foundation
1999-2002 Somatic embryogenesis of Lithospermum erythrorhizon and its secondary metabolite production observed by scanning electron microscopy PI Asahi Glass Foundation
Community Service
  • 1.Totik Sri Mariani; ITB; Pameran Anggrek di MTC Soekarno Hatta Bandung; 3-12 Maret 2006
  • 2.Yusuf H. Ahmad; Dinas Pertanian Maluku; Pembibitan anggrek Grammatophylum scriptum; November 2005
  • 3.Susi Sulistyowati; Asosiasi Petani Anggrek Indonesia cabang Bandung; Pembibitan anggrek Phalaenopsis; April 2006
Kerjasama
  • 1.Morphological Study On Direct Somatic Embryogenesis Of Lithospermum Erythrorhizon And Its Shikonin By Scanning Electron Microscopy; Kyoto University; Kolaborasi; 2000-2002
  • 2.Aplikasi Teknologi Embriogenesis Somatik Dan Embriogenesis Mikrospora Dalam Penyediaan Benih Buatan Kelapa Sawit Untuk Uji Coba Di Lapangan; Nagoya University Kolaborasi; 2005-2007
  • 3.In Depth Study For Developing Efficient Direct Somatic Embryogenesis Of Jatropha Curcas : Morphology And Ultrastructure; Nagoya University; Kolaborasi; 2006-2008
Professional Membership
 International Association for Plant Tissue Culture & Biotechnology 2000-sekarang