Pramesti Istiandari, M.Eng, Ph.D.
Dosen
Sains dan Bioteknologi Tumbuhan
Sekolah ilmu dan teknologi hayati
Institut teknologi bandung
Email : pramesti@itb.ac.id
SINTA | Google Scholar | Scopus
Pramesti Istiandari, M.Eng., Ph.D., mengkhususkan diri pada bioteknologi tanaman dengan fokus pada biosintesis triterpenoid pada tanaman polong-polongan seperti Lotus japonicus dan Abrus precatorius. Penelitiannya menggunakan studi genomik dan transkriptomik, profil metabolit, dan rekayasa genetika menggunakan CRISPR/Cas9. Penelitiannya menekankan pada pembuatan profil senyawa tumbuhan khusus menggunakan GC-MS, khususnya senyawa terpenoid. Dia juga mengeksplorasi rekayasa metabolisme pada Saccharomyces cerevisiae transgenik untuk produksi triterpenoid heterolog. Penelitiannya bertujuan untuk memperdalam pemahaman jalur metabolit sekunder dan memajukan aplikasi bioteknologi untuk produk nabati.
| Prsentasi | |
| 2020 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2020. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. Academic Exchange Seminar Between Shanghai Jiao Tong University and Osaka University, 2nd Industrial Biotechnology Symposium (Online), Japan. |
| 2021 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2021. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. The 62nd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| 2022 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2022. Comparative Analysis of NADPH-Cytochrome P450 Reductase Classes of Lotus japonicus Towards Triterpenoids Biosynthesis. The 63rd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| 2019 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 女子大学院生と企業等との交流会. Osaka, Japan. (Poster Presentation). |
| 2019 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 37th Annual Meeting of the Japanese Society for Plant Cell and Molecular Biology. Kyoto, Japan. |
| Journals & Proceedings |
| Istiandari, P., Yasumoto, S., Seki, H., Fukushima, E. O., & Muranaka, T. (2023). Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus. Frontiers in Plant Science, 14, 1214602. |
| Istiandari, P., Yasumoto, S., Srisawat, P., Tamura, K., Chikugo, A., Suzuki, H., et al. (2021). Comparative analysis of NADPH-cytochrome P450 reductases from legumes for heterologous production of triterpenoids in transgenic Saccharomyces cerevisiae. Frontiers in Plant Science, 12, 762546. |
| Abduh, M. Y., Jamilah, M., Istiandari, P., Manurung, R., & Manurung, R. (2017). Bioconversion of rubber seeds to produce protein and oil-rich biomass using black soldier fly larva assisted by microbes. Journal of Entomology and Zoology Studies, 5(4), 591-597. |
| Presentations |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 37th Annual Meeting of the Japanese Society for Plant Cell and Molecular Biology. Kyoto, Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2020. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. Academic Exchange Seminar Between Shanghai Jiao Tong University and Osaka University, 2nd Industrial Biotechnology Symposium (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2021. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. The 62nd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2022. Comparative Analysis of NADPH-Cytochrome P450 Reductase Classes of Lotus japonicus Towards Triterpenoids Biosynthesis. The 63rd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 女子大学院生と企業等との交流会. Osaka, Japan. (Poster Presentation). |
| Tahun | Nama Kegiatan | Leader | Personil |
| 2024 | EQUIPPING HIGH SCHOOL STUDENTS WITH BIOENTREPRENEURIAL SKILLS THROUGH A YOGURT FERMENTATION WORKSHOP | Ihsan Tria Pramanda | Putu Virgina Partha Devanthi; Mario Donald Bani; Dina Hermawaty; Pramesti Istiandari; Wildan Qoharisma Salam; Riahna Kembaren; |
PROYEK PENELITIAN
| Tahun | Judul Penelitian |
| 2023 | TRANSCRIPTOMIC ANALYSIS OF MEDICINAL LEGUME ABRUS PRECATORIUS TREATED WITH METHYL JASMONATE TO REVEAL THE BIOSYNTHETIC GENES OF ABRUSOSIDE AS A POTENT NATURAL SWEETENER AND THERAPEUTIC AGENT |
| 2024 | ENHANCING THE PRODUCTION OF THERAPEUTIC TRITERPENOID SAPONINS IN SAGA (ABRUS PRECATORIUS) THROUGH HYDROPONIC CULTIVATION |
| 2024 | MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERIZATION OF PLANT GROWTH–PROMOTING MICROBES FROM COMPOST-TREATED SOIL AND PLANT |
| 2024 | Optimasi metode kultur jaringan tanaman sacha inchi (Plukenetia volubilis. L) yang berkelanjutan dan resilien terhadap perubahan iklim |
HIBAH PENELITAN
| Optimizing Tissue Culture Methods for the Propagation of Sacha Inchi (Plukenetia volubilis L.) as a Sustainable and Climate-Resilient Functional Food Source. Ministry of Research and Education. 2024. |
| Unveiling High-Value Triterpenoid Biosynthesis in Medicinal Legume Abrus precatorius Through Transcriptomic Analysis and Methyl Jasmonate Treatment. Toray Science and Technology Research Grant. 2023. |
| Enhancing the Production of Therapeutic Triterpenoid Saponins in Saga (Abrus precatorius) through Hydroponic Cultivation. Indonesia International Institute for Life Sciences Internal Grant. 2023. |
| Tahun | Nama Mata Kuliah |
| 2024/2025 | Neraca Massa dan Energi Sistem Hayati |
| 2024/2025 | Kapita Selekta Biologi |
| 2024/2025 | Pengantar Rekayasa Hayati |
| 2024/2025 | Rekayasa Bioproduk |
| 2024/2025 | Rekayasa Sel dan Jaringan Tanaman |
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