The application of self-eDNA in agriculture presents the potential to increase the resistance of banana plantlets. Karlia Meitha, Ph.D., Dr. Iriawati, Dr. Rizkita Rachmi Esyanti, and colleagues conducted this study that evaluated whether the eDNA from Fusarium oxysporum f. sp. cubense (Foc) could limit the growth of Foc itself while increasing the resistance of banana plant. A self-inhibition test showed that the administration of 400 and 800 μg mL−1 eDNA inhibited Foc TR4 spore germination. After 24 h, the plantlets were infected with a suspension of 106 spore mL−1. Increased resistance was observed in plantlets treated with 200 μg mL−1 of Foc TR4 eDNA, as indicated by the decrease in leaf symptoms and rhizome discoloration. The accumulation of O2- and H2O2 was observed 24 h after priming as was a significant increase in the relative expression of CAT, PR1, and chitinase 1 genes on day 9 post-infection. In conclusion, eDNA Foc TR4 as a growth inhibitor of pathogen and a priming agent to the banana plantlets could be considered as a biofungicide candidate to induce the resistance of banana plants.

Article Citation:
Meitha, K., Hanisia, R. H., Signorelli, S., Fauziah, T., Iriawati, Esyanti, R. R. (2023). Extracellular DNA of Fusarium oxysporum f. sp. Cubense as a Priming Agent for Inducing the Resistance of Banana Plantlets. Agronomy; 13 (2), 441. https://doi.org/10.3390/agronomy13020441

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Profile of Karlia Meitha, Ph.D.:
https://sith.itb.ac.id/karlia-meitha-ph-d-2/

Profile of Dr. Iriawati:
https://sith.itb.ac.id/dr-iriawati/

Profile of Dr. Rizkita Rachmi Esyanti:
https://sith.itb.ac.id/dr-rizkita-rachmi-esyanti/