Plant-derived exosome-like nanoparticles (PDENs) are a promising candidate for therapeutic application because they are safe, biodegradable and biocompatible. In this study, papaya PDENs were isolated by a PEG6000-based method and characterized by dynamic light scattering (DLS), transmission Electron Microscopy (TEM), bicinchoninic acid (BCA) assay method, GC-MS analysis, total phenolic content (TPC) analysis, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. For the in vitro test, Dr. Iriawati, M.Sc., Prof. Dr. Anggraini Barlian, M.Sc., and their colleagues conducted internalization analysis, toxicity assessment, determination of nitrite concentration, and assessed the expression of inflammatory cytokine genes using qRT-PCR in RAW 264.7 cells. For the in vivo test, inflammation was induced by caudal fin amputation followed by analysis of macrophage and neutrophil migration in zebrafish (Danio rerio) larvae. The result showed that papaya PDENs possesses anti-inflammatory properties.

Article Citation:
Iriawati, Vitasasti, S., Rahmadian, F.N.A., Barlian, A. (2024) Isolation and characterization of plant-derived exosome-like nanoparticles from Carica papaya L. fruit and their potential as antiinflammatory agent. PLoS ONE 19(7): e0304335. https://doi.org/10.1371/journal.pone.0304335