KARLIA MEITHA, S.Si., M.Si., Ph.d.
Assistant Professor
School of Life Sciences and Technology
Institut Teknologi Bandung, Indonesia
Expertise Group : Genetics and Molecular Biotechnology
Email : karliameitha@itb.ac.id
Overview
Karlia Meitha, S.Si., M.Si., Ph.D. primarily focuses in Genetics an Molecular Biotechnology, specifically in the research of genome editing; small-RNAs for biocontrol; epigenetics; and high-antioxidant production in crops.
Bachelor’s Degree
- Institut Teknologi Bandung, Indonesia (2006).
Master’s Degree
- Institut Teknologi Bandung, Indonesia (2010).
Doctoral Degree
- Universiy of Western Australia, Australia (2017).
Undergraduate Courses
- Genetics; Syntetic Biology; Fundamentals for Protein Engineering;
- Cellular and Molecular Biology; Microbial Genetics and Genetic Engineering
Postgraduate Courses
- Advanced Molecular Genetics; Biotechnology of Postharvest; Plant Stress and Biotechnology
- Research Design, Bioethics, and Patent; Biotechnology Research and Seminar; Thesis and Final Defense
- Meitha, K., Chen, P. Y., Chang, C., Lin, Z. C., Hsu, T. W., Gojobori, T., & Chiang, T. Y. (2024). Is an invasive species a notorious invader or carbon sequencer?. Ecological Genetics and Genomics, 100279.
- Hermawaty, D., Meitha, K., & Esyanti, R. R. (2024). The Potential of Methylation Sensitive Amplification Polymorphism (MSAP) Technique to Study Global DNA Methylation Changes in Plants: Basic Principles and Perspective. Reviews in Agricultural Science, 12, 93-110.
- Septiani, P., Pramesti, Y., Ningsih, D. U., Pancaningtyas, S., & Meitha, K. (2024). Identification of self-and pathogen-targeted miRNAs from resistant and susceptible Theobroma cacao variety to black pod disease. Scientific Reports, 14(1), 3272.
- Tham, J., Patisenah, A., Tjia, T. O. S., Signorelli, S., Taufik, I., & Meitha, K. (2023). sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐nuum L. Indonesian Journal of Biotechnology, 28(4), 238-247.
- Meitha, K., Hanisia, R. H., Signorelli, S., Fauziah, T., Iriawati, and Esyanti, R. R. (2023) Extracellular DNA of Fusarium oxysporum sp. cubense as a priming agent for inducing the resistance of banana plantlets. Agronomy. 13(2:441). https://doi.org/10.3390/agronomy13020441
- Wiprayoga, I., Meitha, K., and Dwivany, F.M. (2023) In Silico Characterization of Lycopene Beta Cyclase (LCYB) and Lycopene Epsilon Cyclase (LCYE) Genes from DH-Pahang (Musa acuminata, A Genome) and DH-PKW (Musa balbisiana, B Genome). Journal of Tropical Lofe Sciences. 13(1). https://doi.org/10.11594/jtls.13.01.09
- Tjia, T. O. S., Meitha, K., Septiani, P., Awaludin, R., and Sumardi, D. (2023) Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots. 67, 9-18. DOI: 10.32615/bp.2022.037
- Meitha, K., Pramesti, Y., Signorelli, S., and Kriswantoro, J.A. (2022) Postharvest chitosan application maintains the quality of spinach through suppression of bacterial growth and elicitation. Horticulture, Environment, and Biotechnology. 63(2), 217-227. https://doi.org/10.1007/s13580-021-00397-0
- Suhandono, S., Kusuma, S. H., Meitha, K. (2021) Characterization and Production of Rhamnolipid Biosurfactant in Recombinant Escherichia coli Using Autoinduction Medium and Palm Oil Mill Effluent. Brazilian Archives of Biology and Technology. https://doi.org/10.1590/1678-4324-2021200301
- Meitha, K., Esyanti, R. R., and Hanisia, R. H. (2021) Green pesticide: Tapping to the promising roles of plant secreted small RNAs and responses towards extracellular DNA. Non-coding RNA Research. 6(1). 42-50. https://doi.org/10.1016/j.ncrna.2021.02.001
- Setiabudi, E., Meitha, K., and Dwivany, F. M. (2021) In silico characterization and comparison of the fruit ripening related beta‐amylase (BAM) gene family in banana genome A and B. Indonesian Journal of Biotechnology. 26(4). https://doi.org/10.22146/ijbiotech.65142
- Kusuma, S. H., Meitha, K., Suhandono, S. (2021) Characterization of Di-Rhamnolipid Biosurfactant in Recombinant Escherichia coli. Key Engineering Materials.https://doi.org/10.4028/www.scientific.net/KEM.874.107
- Dwivany, F. M., Sukriandi, N., Meitha, K., Brotosudarmo, T. H. P. (2021) In silico Characterization of the Structure of Genes and Proteins related to β-carotene Degradation in Musa acuminata ‘DHPahang’ and Musa balbisiana’Pisang Klutuk Wulung’. Pertanika Journal of Tropical Agricultural Science. 44(2). DOI: https://doi.org/10.47836/pjtas.44.2.10
- Nugrahapraja, H. N., Dwivany, F. M., Meitha, K., Kuswati, Esyanti, R. R. (2021) Chitosan improving Growth in Chili (Capsicum annuum L.) Plants and acting through Distinct Gene Regulation between Cultivars. Research Journal of Biotechnology. 16(2)
- K. Pramesti, Y., Suhandono, S. (2020) Reactive oxygen species and antioxidants in postharvest vegetables and fruits. International journal of food science. https://doi.org/10.1155/2020/8817778
- Meitha, K., Fatmawati, I., Dwivany, F.M., Sutanto, A., Pratama, S.N., Nugrahapraja, H., and Wikantika, K. (2020) Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.49506
- Aziz, M.A., Esyanti, R.R., Meitha, K., Dwivany, F.M., and Chotimah, H.H. (2020) Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.55016
- Dwivany, F. M., Ramadhan, M. R., Lim, C., Sutanto, A., Nugrahapraja, H., Wikantika, K., Pratama, S. N., Meitha, K., Pratiwi, A. S. (2020). Bali Bananas (Musa spp. L.) Genetic Relationship Based on Internal Transcribed Spacer 2 (ITS-2). Pertanika Journal of Tropical Agricultural Science. 43(4)
- Esyanti, R.E., Dwivany, F. M., Mahani, S., Nugrahapraja, H. & Meitha, K. (2019) Foliar application of chitosan enhances growth and modulates expression of defense genes in chilli pepper (Capsicum annuum L.). Australian Journal of Crop Sciences. 13(01). doi: 10.21475/ajcs.19.13.01.p1169
- Meitha, K., Agudelo-Romero, P., Signorelli, S., Considine, J., Foyer, C. & Considine, M. (2018) Developmental Control of Hypoxia During Bud Burst in Grapevine. Plant, Cell, and Environment. doi:10.1111/pce.13141
- Signorelli, S., Agudelo-Romero, P., Meitha, K., Foyer, C. H., & Considine, M. J. (2018) Roles for Light, Energy, and Oxygen in the Fate of Quiescent Axillary Buds. Plant Physiology. doi: 10.1104/pp.17.01479
- Rai, I N., Dwivany, F. M., Sutanto, A., Meitha, K., Sukewijaya, I M., and Ustriyana, I N. G. (2018) Biodiversity of Bali Banana (Musaceae) and its Usefulness. Hayati Journal of Biosciences. 25 (2). doi: 10.4308/hjb.25.2.47
- Hidayat, T., Kelana, H.W., Ismanto, D.I.A., and Meitha, K. (2018) Survey on Ethnobotanic Value of Banana (Musa spp; Musaceae) in Bali Province, Indonesia. Hayati Journal of Biosciences. 25 (1). doi:10.4308/hjb/25.1.31
- Shaw, D. S., Meitha, K., Considine, M. J., & Foyer, C. H. (2017). Mitochondrial Respiration and Oxygen Tension. In K. Jagadis Gupta (Ed.), Plant Respiration and Internal Oxygen: Methods and Protocols (pp. 97-113). New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-7292-0_11
- Meitha, K. & Considine, M. (2017) Regulation of Oxygen and Reactive Oxygen Species During Budburst. Wine and Viticulture Journal. 32:4. (pp. 42-45)
- Meitha, K. (2016). Promotor Gen: Tombol On/Off Ekspresi Gen yang Penting Bagi Kelangsungan Hidup Organisme. In Ketut Wikantika (Ed.), Bunga Rampai Forum Peneliti Muda Indonesia (pp. 9-14). Bandung: Penerbit ITB. ISBN: 978-602-7861-52-7
- Meitha, K., Konnerup, D., Colmer, T. D., Considine, J. A., Foyer, C. H., & Considine, M. J. (2015). Spatio-temporal relief from hypoxia and production of reactive oxygen species during bud burst in grapevine (Vitis vinifera). Annals of Botany (Reactive Oxygen and Nitrogen Species). doi:10.1093/aob/mcv123
Professional membership
- International Society for Horticultural Science, 2022 – present
- Asian Federation of Biotechnology (Indonesia Chapter), 2022 – present
- Indonesian Biotechnology Program Association, 2020 – present
- Society of Experimental Biology, London, United Kingdom, 2013 – present
Awards
Research visit to Dr. Keiji Nishida laboratory at Kobe University, Japan |
Nov 2022 – Feb 2023
|
Granted by the Rector of ITB at the 63rd Dies Natalis |
Feb 2022 |
The 5th International Biology Conference, Biology Department, ITS – Indonesia |
October 2020 |
To attend annual main meeting in Prague, Czech. |
Jun – Jul 2015 |
Research visit to Prof. Christine Foyer laboratory at Leeds University. |
Jun – Sep 2013 |
The University of Western Australia. |
Jun 2012 – Jul 2016 |
Udayana University, Bali – Indonesia. |
Sep 2009 |
Grants
1. Riset Kolaboratif antar KK- ITB
Edit Genome of MiRNA Template Regions in Chili to Increase Resistance to Phytophthora capsici |
Mar – Nov 2023
|
2. Riset Peningkatan Kapasitas KK – ITB
Global Analysis of DNA Methylation for Postharvest Management Development |
Mar – Nov 2023
|
3. Riset Unggulan – ITB
Development of Highly Soluble Cas9 Synthesis System for Transgenic Free CRISPR-Cas9 Genome Engineering |
Feb – Nov 2022
|
4. PPMI KK GBM – SITH ITB
Identification of Theobroma cacao miRNA as Biopesticide Candidate for Phytophthora palmivora |
Feb – Nov 2022 |
5. Riset Unggulan – ITB
Development of Potyvirus Resistant Chili (Capsicum annuum) through Modification of the eIF4E Gene using the CRISPR-Cas9 Genome Editing Method |
Feb – Nov 2021 |
6. Riset Inovasi LPiK – ITB
Optimization of the manufacture of Inactivated Virus Transport Media (VTM) |
Feb – Nov 2021 |
7. Research Grant (PT. Rekacipta Inovasi – ITB)
Recovery Rate Determination of Activated Virus Transport Medium |
Feb – Nov 2021 |
8. Research Grant (LPiK – ITB)
The preparation of viral transport media for local detetion center in Bandung |
May – Oct 2020 |
9. Riset peningkatan kapasitas – ITB
Improving antioxidant contents in microgreens by combination of red and blue light 10. Research Grant P3MI – ITB Effect of Chitosan Coating on the Postharvest Quality and Regulation of Gene Expression Related to ROS (Reactive Oxygen Species) od Spinach (Spinacia oleracea) |
Feb – Nov 2020
Feb – Nov 2019 |
The Research Foundation of Indonesia Biogeography and Biodiversity Director, Bandung, Indonesia Dec 2017 – 2020 |
Plan, lead, and review organisational development strategies with the board of directors |
Bali International Research Centre for Banana SecretaryBali, Indonesia Feb 2017 – 2020 |
Facilitate the communication between two main universities involved in the research, ITB and Udayana University; Managing research activities. |
The Association of Indonesian Postgraduate Students and Scholars in Australia Vice President Treasurer Perth, Australia Jan – Dec 2015 Jan – Dec 2014 |
Collaborated with other community organizations in Perth to create friendship events in sports and women in leadership.Promoted and managed fund-raising projects.Informed future Indonesian students about education systems and life in Australia. |
Institut Teknologi Bandung Lecturer and Researcher Bandung, Indonesia Jan 2018 – Present |
· Facilitate students to grasp the knowledge in Biology topics and related research skills
· Conduct research in the field of plant biomolecular and its application |
Institut Teknologi Bandung Bandung, Indonesia Academic Assistant and Postdoctoral Fellow Oct 2016 – Dec 2017 |
· Conducted biomolecular research in the topics of banana diversity.
· Assisted the curriculum evaluation process and preparation of new curriculum documents for Microbiology |
The University of Western Australia Demonstrator for laboratory practice Perth, Australia Aug – Nov 2014 |
· Guided undergraduate students to perform essential physiology and molecular experiments.
· Helped the students to understand crucial concepts underlying the experiments. |
Leeds University Leeds, UK Visiting researcher Jun 2013 – Sep 2013 |
· Observed the localization of reactive oxygen species in grape bud tissues by using histological method.
· Examined techniques for mitochondria extraction and oxygen consumption rate measurements. |