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KARLIA MEITHA, S.Si.,M.Si., Ph.D.

KARLIA MEITHA, S.Si., M.Si., Ph.d.

Assistant Professor
School of Life Sciences and Technology
Institut Teknologi Bandung, Indonesia
Expertise Group : Genetics and Molecular Biotechnology
Email : karliameitha@itb.ac.id

Overview

Karlia Meitha, S.Si., M.Si., Ph.D. primarily focuses in Genetics an Molecular Biotechnology, specifically in the research of genome editing; small-RNAs for biocontrol; epigenetics; and high-antioxidant production in crops.

Bachelor’s Degree

  • Institut Teknologi Bandung, Indonesia (2006).

Master’s Degree

  • Institut Teknologi Bandung, Indonesia (2010).

Doctoral Degree

  • Universiy of Western Australia, Australia (2017).

Undergraduate Courses

  • Genetics; Syntetic Biology; Fundamentals for Protein Engineering;
  • Cellular and Molecular Biology; Microbial Genetics and Genetic Engineering

Postgraduate Courses

  • Advanced Molecular Genetics; Biotechnology of Postharvest; Plant Stress and Biotechnology
  • Research Design, Bioethics, and Patent; Biotechnology Research and Seminar; Thesis and Final Defense
  1. Meitha, K., Chen, P. Y., Chang, C., Lin, Z. C., Hsu, T. W., Gojobori, T., & Chiang, T. Y. (2024). Is an invasive species a notorious invader or carbon sequencer?. Ecological Genetics and Genomics, 100279.
  2. Hermawaty, D., Meitha, K., & Esyanti, R. R. (2024). The Potential of Methylation Sensitive Amplification Polymorphism (MSAP) Technique to Study Global DNA Methylation Changes in Plants: Basic Principles and Perspective. Reviews in Agricultural Science, 12, 93-110.
  3. Septiani, P., Pramesti, Y., Ningsih, D. U., Pancaningtyas, S., & Meitha, K. (2024). Identification of self-and pathogen-targeted miRNAs from resistant and susceptible Theobroma cacao variety to black pod disease. Scientific Reports, 14(1), 3272.
  4. Tham, J., Patisenah, A., Tjia, T. O. S., Signorelli, S., Taufik, I., & Meitha, K. (2023). sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐nuum L. Indonesian Journal of Biotechnology, 28(4), 238-247.
  5. Meitha, K., Hanisia, R. H., Signorelli, S., Fauziah, T., Iriawati, and Esyanti, R. R. (2023) Extracellular DNA of Fusarium oxysporum sp. cubense as a priming agent for inducing the resistance of banana plantlets. Agronomy. 13(2:441). https://doi.org/10.3390/agronomy13020441
  6. Wiprayoga, I., Meitha, K., and Dwivany, F.M. (2023) In Silico Characterization of Lycopene Beta Cyclase (LCYB) and Lycopene Epsilon Cyclase (LCYE) Genes from DH-Pahang (Musa acuminata, A Genome) and DH-PKW (Musa balbisiana, B Genome). Journal of Tropical Lofe Sciences. 13(1). https://doi.org/10.11594/jtls.13.01.09
  7. Tjia, T. O. S., Meitha, K., Septiani, P., Awaludin, R., and Sumardi, D. (2023) Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots. 67, 9-18. DOI: 10.32615/bp.2022.037
  8. Meitha, K., Pramesti, Y., Signorelli, S., and Kriswantoro, J.A. (2022) Postharvest chitosan application maintains the quality of spinach through suppression of bacterial growth and elicitation. Horticulture, Environment, and Biotechnology. 63(2), 217-227. https://doi.org/10.1007/s13580-021-00397-0
  9. Suhandono, S., Kusuma, S. H., Meitha, K. (2021) Characterization and Production of Rhamnolipid Biosurfactant in Recombinant Escherichia coli Using Autoinduction Medium and Palm Oil Mill Effluent. Brazilian Archives of Biology and Technology. https://doi.org/10.1590/1678-4324-2021200301
  10. Meitha, K., Esyanti, R. R., and Hanisia, R. H. (2021) Green pesticide: Tapping to the promising roles of plant secreted small RNAs and responses towards extracellular DNA. Non-coding RNA Research. 6(1). 42-50. https://doi.org/10.1016/j.ncrna.2021.02.001
  11. Setiabudi, E., Meitha, K., and Dwivany, F. M. (2021) In silico characterization and comparison of the fruit ripening related beta‐amylase (BAM) gene family in banana genome A and B. Indonesian Journal of Biotechnology. 26(4). https://doi.org/10.22146/ijbiotech.65142
  12. Kusuma, S. H., Meitha, K., Suhandono, S. (2021) Characterization of Di-Rhamnolipid Biosurfactant in Recombinant Escherichia coli. Key Engineering Materials.https://doi.org/10.4028/www.scientific.net/KEM.874.107
  13. Dwivany, F. M., Sukriandi, N., Meitha, K., Brotosudarmo, T. H. P. (2021) In silico Characterization of the Structure of Genes and Proteins related to β-carotene Degradation in Musa acuminata ‘DHPahang’ and Musa balbisiana’Pisang Klutuk Wulung’. Pertanika Journal of Tropical Agricultural Science. 44(2). DOI: https://doi.org/10.47836/pjtas.44.2.10
  14. Nugrahapraja, H. N., Dwivany, F. M., Meitha, K., Kuswati, Esyanti, R. R. (2021) Chitosan improving Growth in Chili (Capsicum annuum L.) Plants and acting through Distinct Gene Regulation between Cultivars. Research Journal of Biotechnology. 16(2)
  15. K. Pramesti, Y., Suhandono, S. (2020) Reactive oxygen species and antioxidants in postharvest vegetables and fruits. International journal of food science. https://doi.org/10.1155/2020/8817778
  16. Meitha, K., Fatmawati, I., Dwivany, F.M., Sutanto, A., Pratama, S.N., Nugrahapraja, H., and Wikantika, K. (2020) Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.49506
  17. Aziz, M.A., Esyanti, R.R., Meitha, K., Dwivany, F.M., and Chotimah, H.H. (2020) Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.55016
  18. Dwivany, F. M., Ramadhan, M. R., Lim, C., Sutanto, A., Nugrahapraja, H., Wikantika, K., Pratama, S. N., Meitha, K., Pratiwi, A. S. (2020). Bali Bananas (Musa spp. L.) Genetic Relationship Based on Internal Transcribed Spacer 2 (ITS-2). Pertanika Journal of Tropical Agricultural Science. 43(4)
  19. Esyanti, R.E., Dwivany, F. M., Mahani, S., Nugrahapraja, H. & Meitha, K. (2019) Foliar application of chitosan enhances growth and modulates expression of defense genes in chilli pepper (Capsicum annuum L.). Australian Journal of Crop Sciences. 13(01). doi: 10.21475/ajcs.19.13.01.p1169
  20. Meitha, K., Agudelo-Romero, P., Signorelli, S., Considine, J., Foyer, C. & Considine, M. (2018) Developmental Control of Hypoxia During Bud Burst in Grapevine. Plant, Cell, and Environment. doi:10.1111/pce.13141
  21. Signorelli, S., Agudelo-Romero, P., Meitha, K., Foyer, C. H., & Considine, M. J. (2018) Roles for Light, Energy, and Oxygen in the Fate of Quiescent Axillary Buds. Plant Physiology. doi: 10.1104/pp.17.01479
  22. Rai, I N., Dwivany, F. M., Sutanto, A., Meitha, K., Sukewijaya, I M., and Ustriyana, I N. G. (2018) Biodiversity of Bali Banana (Musaceae) and its Usefulness. Hayati Journal of Biosciences. 25 (2). doi: 10.4308/hjb.25.2.47
  23. Hidayat, T., Kelana, H.W., Ismanto, D.I.A., and Meitha, K. (2018) Survey on Ethnobotanic Value of Banana (Musa spp; Musaceae) in Bali Province, Indonesia. Hayati Journal of Biosciences. 25 (1). doi:10.4308/hjb/25.1.31
  24. Shaw, D. S., Meitha, K., Considine, M. J., & Foyer, C. H. (2017). Mitochondrial Respiration and Oxygen Tension. In K. Jagadis Gupta (Ed.), Plant Respiration and Internal Oxygen: Methods and Protocols (pp. 97-113). New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-7292-0_11
  25. Meitha, K. & Considine, M. (2017) Regulation of Oxygen and Reactive Oxygen Species During Budburst. Wine and Viticulture Journal. 32:4. (pp. 42-45)
  26. Meitha, K. (2016). Promotor Gen: Tombol On/Off Ekspresi Gen yang Penting Bagi Kelangsungan Hidup Organisme. In Ketut Wikantika (Ed.), Bunga Rampai Forum Peneliti Muda Indonesia (pp. 9-14). Bandung: Penerbit ITB. ISBN: 978-602-7861-52-7
  27. Meitha, K., Konnerup, D., Colmer, T. D., Considine, J. A., Foyer, C. H., & Considine, M. J. (2015). Spatio-temporal relief from hypoxia and production of reactive oxygen species during bud burst in grapevine (Vitis vinifera). Annals of Botany (Reactive Oxygen and Nitrogen Species). doi:10.1093/aob/mcv123

Professional membership

  • International Society for Horticultural Science, 2022 – present
  • Asian Federation of Biotechnology (Indonesia Chapter), 2022 – present
  • Indonesian Biotechnology Program Association, 2020 – present
  • Society of Experimental Biology, London, United Kingdom, 2013 – present

Awards

  • The Matsumae International Fellowship

Research visit to Dr. Keiji Nishida laboratory at Kobe University, Japan

Nov 2022 – Feb 2023

 

  • Award in Teaching Excellence

Granted by the Rector of ITB at the 63rd Dies Natalis

Feb 2022
  • The Best Paper Presenter

The 5th International Biology Conference, Biology Department, ITS – Indonesia

October 2020
  • Travel Grant from Society for Experimental Biology

To attend annual main meeting in Prague, Czech.

Jun – Jul 2015
  • Travel Grant from Grape and Wine Research Centre Australia

Research visit to Prof. Christine Foyer laboratory at Leeds University.

Jun – Sep 2013
  • PhD Scholarship from Australia Awards

The University of Western Australia.

Jun 2012 – Jul 2016
  • Best Oral Presenter at International Seminar “Biotechnology for A Sustainable Future”

Udayana University, Bali – Indonesia.

 Sep 2009

 

Grants

1.      Riset Kolaboratif antar KK- ITB

Edit Genome of MiRNA Template Regions in Chili to Increase Resistance to Phytophthora capsici

Mar – Nov 2023

 

2.      Riset Peningkatan Kapasitas KK – ITB

Global Analysis of DNA Methylation for Postharvest Management Development

Mar – Nov 2023

 

3.      Riset Unggulan – ITB

Development of Highly Soluble Cas9 Synthesis System for Transgenic Free CRISPR-Cas9 Genome Engineering

Feb – Nov 2022

 

4.      PPMI KK GBM – SITH ITB

Identification of Theobroma cacao miRNA as Biopesticide Candidate for Phytophthora palmivora

Feb – Nov 2022
5.      Riset Unggulan – ITB

Development of Potyvirus Resistant Chili (Capsicum annuum) through Modification of the eIF4E Gene using the CRISPR-Cas9 Genome Editing Method

Feb – Nov 2021
6.      Riset Inovasi LPiK – ITB

Optimization of the manufacture of Inactivated Virus Transport Media (VTM)

Feb – Nov 2021
7.      Research Grant (PT. Rekacipta Inovasi – ITB)

Recovery Rate Determination of Activated Virus Transport Medium

Feb – Nov 2021
8.      Research Grant (LPiK – ITB)

The preparation of viral transport media for local detetion center in Bandung

May – Oct 2020
9.      Riset peningkatan kapasitas – ITB

Improving antioxidant contents in microgreens by combination of red and blue light

10.  Research Grant P3MI – ITB

Effect of Chitosan Coating on the Postharvest Quality and Regulation of Gene Expression Related to ROS (Reactive Oxygen Species) od Spinach (Spinacia oleracea)

Feb – Nov 2020

 

 

Feb – Nov 2019

The Research Foundation of Indonesia Biogeography and Biodiversity Director, Bandung, Indonesia
Dec 2017 – 2020
Plan, lead, and review organisational development strategies with the board of directors
Bali International Research Centre for Banana
SecretaryBali, Indonesia
Feb 2017 – 2020
Facilitate the communication between two main universities involved in the research, ITB and Udayana University; Managing research activities.
The Association of Indonesian Postgraduate Students and Scholars in Australia Vice President
Treasurer Perth, Australia
Jan – Dec 2015
Jan – Dec 2014
Collaborated with other community organizations in Perth to create friendship events in sports and
women in leadership.Promoted and managed fund-raising projects.Informed future Indonesian students about education systems and life in Australia.
Institut Teknologi Bandung
Lecturer and Researcher
Bandung, Indonesia
Jan 2018 – Present
· Facilitate students to grasp the knowledge in Biology topics and related research skills

· Conduct research in the field of plant biomolecular and its application

Institut Teknologi Bandung Bandung, Indonesia
Academic Assistant and Postdoctoral Fellow Oct 2016 – Dec 2017
· Conducted biomolecular research in the topics of banana diversity.

· Assisted the curriculum evaluation process and preparation of new curriculum documents for Microbiology
and Biotechnology study programs.

The University of Western Australia
Demonstrator for laboratory practice
Perth, Australia
Aug – Nov 2014
· Guided undergraduate students to perform essential physiology and molecular experiments.

· Helped the students to understand crucial concepts underlying the experiments.

Leeds University Leeds, UK
Visiting researcher Jun 2013 – Sep 2013
· Observed the localization of reactive oxygen species in grape bud tissues by using histological method.

· Examined techniques for mitochondria extraction and oxygen consumption rate measurements.

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