HUSNA NUGRAHAPRAJA, PH.D.
Assistant Professor
Genetics and Molecular Biotechnology
School of Sciences and Technology
Bandung Institut of Technology
Email : husna_np@itb.ac.id
SINTA | Google Scholar | Scopus
OVERVIEW
Husna Nugrahapraja, Ph.Dcompleted his doctoral program with a thesis entitled “Genetic and molecular analysis of the cross-incompatibility (CI) phenomenon in maize” from Scuola Superiore Sant’Anna Pisa, Italy, in 2015. Currently, his research focused on data analysis molecular data derived from Next-Generation Sequencing (NGS), Genomics and Transcriptomics, and Identification and Characterization of lncRNA molecules (long non-coding RNA). His primary expertises include NGS Big Data Analytics, Omics and Sequencing Analysis, Bioinformatics, Genetics, and Molecular Biotechnology.
Doctoral degree
- Scuola Superiore Sant’Anna Pisa, Italy (2015).
| Novianti, C., Sari, L. D. N., Nugrahapraja, H., Suhandono, S., Dwivany, F. M., Putri, S. P., & Fukusaki, E. (2025). Metabolic profiling reveals distinctive ripening dynamics in ethylene-treated Musa balbisiana cv. ‘Pisang Klutuk Wulung’ compared to commercial Cavendish banana. Journal of Bioscience and Bioengineering. https://doi.org/10.1016/j.jbiosc.2025.01.001 |
| Fibriani, A., Yamahoki, N., Shani, A. M., Rofiqoh, A., Siregar, A. L. F., Gunawan, C., Mayorga, C. G. D., Tjia, T. O. S., Nugrahapraja, H., Giri-Rachman, E. A., & Tan, M. I. (2025). Reverse vaccinology-based multi-epitope COVID-19 vaccine targeting SARS-CoV-2 structural and non-structural proteins induces immune responses in mice. Vaccine X, 100632. https://doi.org/10.1016/j.jvacx.2025.100632 |
| Dwivany, F. M., Nurdin, I. I. L., Getas, K. a. G., Takalamingan, H. N., Putri, R. R., Farahyah, J. D., Wicaksono, A., Moeis, M. R., Nugrahapraja, H., & Radjasa, O. K. (2025). Metatranscriptomic analysis of chitosan-coated fruit reveals dynamics in active microbiomes during banana ripening. Horticulture Environment and Biotechnology. https://doi.org/10.1007/s13580-025-00682-2 |
| Widiasta, A., Sribudiani, Y., Nugrahapraja, H., & Rachmadi, D. (2025). miRNAs involved in the TGFB signaling as possible markers of steroid-resistant nephrotic syndrome in children. Gene Reports, 102173. https://doi.org/10.1016/j.genrep.2025.102173 |
| Hermawaty, D., Setyobudi, T., Nugrahapraja, H., Turjaman, M., & Faizal, A. (2025). De novo transcriptome assembly and analysis during agarwood induction in Gyrinops versteegii Gilg. seedling. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-87486-7 |
| Dwivany, F. M., Fauziah, T., Yamamoto, K., Novianti, C., Perwira, K. P. C., Rizanti, M., Radjasa, S. K., Hakim, F. S., Salim, A. S. P., Putri, R. R., Wicaksono, A., Sumardi, D., Putri, S. P., Fukusaki, E., Meitha, K., & Nugrahapraja, H. (2024). Chitosan coating to delay the ripening process in banana: a transcriptomics study. Horticulture Environment and Biotechnology, 66(1), 123–136. https://doi.org/10.1007/s13580-024-00642-2 |
| Purwestri, Y. A., Nuringtyas, T. R., Wibowo, A. T., Nugrahapraja, H., Salsinha, Y. C. F., Sebastian, A., Nurbaiti, S., Kumalasari, N., Annisa, R. R., Putri, S. P. M., Az-Zahra, S., & Umami, L. A. (2024). Application of osmoprotectant enhance tolerance to drought stress in rice and trigger changes in root microbial composition. Journal of Plant Biochemistry and Biotechnology. https://doi.org/10.1007/s13562-024-00933-w |
| Radjasa, O. K., Steven, R., Natanael, Y., Nugrahapraja, H., Radjasa, S. K., Kristianti, T., Moeis, M. R., Trinugroho, J. P., Suharya, H. B., Rachmatsyah, A. O., Dwijayanti, A., Putri, M. R., De Fretes, C. E., Siallagan, Z. L., Fadli, M., Opier, R. D. A., Farahyah, J. D., Rahmawati, V., Rizanti, M., . . . Dwivany, F. M. (2024). From the depths of the Java Trench: genomic analysis of Priestia flexa JT4 reveals bioprospecting and lycopene production potential. BMC Genomics, 25(1). https://doi.org/10.1186/s12864-024-11115-2 |
| Nugrahapraja, H., Syam, F., Momole, J., Meitha, K., Wicaksono, A., Moeis, M. R., Radjasa, O. K., & Dwivany, F. M. (2024). Response of the Microbial Community in Unripe and Ripe Bananas with Chitosan Treatment to Delay Fruit Ripening. Agricultural Research. https://doi.org/10.1007/s40003-024-00817-4 |
| Verdiani, H., Syafira, D., & Nugrahapraja, H. (2023). MINI REVIEW: PENGEMBANGAN BIOSENSOR KOLORIMETRI BERBASIS AGREGASI NANOPARTIKEL EMAS. KLOROFIL Jurnal Ilmu Biologi Dan Terapan, 7(1), 1. https://doi.org/10.30821/kfl:jibt.v7i1.14657 |
| Wilbert, N., & Nugrahapraja, N. H. (2023). Diversity analysis of moray eel (muraenidae) on artificial reef structure in mengiat beach, Nusa Dua, Bali. Environmental and Materials, 1(1). https://doi.org/10.61511/eam.v1i1.2023.62 |
| Angellia, V., & Nugrahapraja, N. H. (2023). Study of the sea urchins (echinoidea) influence on the coral reef communities in the Nusa Dua Bali conservation area. Environmental and Materials, 1(1). https://doi.org/10.61511/eam.v1i1.2023.63 |
| Putra, A. M., Nugrahapraja, H., & Dwivany, F. M. (2023). Revealing disease‐specific endogenous target mimic of microRNA from long non‐coding RNA identification and characterization in Musa spp. Indonesian Journal of Biotechnology, 28(1), 56.a https://doi.org/10.22146/ijbiotech.49368 |
| Meitha, K., Fatmawati, I., Dwivany, F. M., Sutanto, A., Pratama, S. N., Nugrahapraja, H., & Wikantika, K. (2020). Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region. Indonesian Journal of Biotechnology, 25(1), 1. https://doi.org/10.22146/ijbiotech.49506 |
| · Dwivany, F. M., Stefani, G., Sutanto, A., Nugrahapraja, H., Wikantika, K., Hiariej, A., Hidayat, T., Rai, I. N., & Sukriandi, N. (2020). Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2. HAYATI Journal of Biosciences, 27(3), 258. https://doi.org/10.4308/hjb.27.3.258 |
| · Dwivany, F.M., Nugrahapraja, H., Rahmawati, A.R., Meitasari, D.P., Putra, A.M., Septiani, P., Farah, N., Subandiyah, S. 2020. Data on banana transcriptome in response to blood disease infection. Data in Brief. Volume 29 |
| · Dwivany, F.M., Syam, F., Nugrahapraja, H., Radjasa, O.K., Moeis, M.R., Uchiyama, S. 2020. Dataset of microbial community structure in alcohol sprayed banana associated with ripening process. Data in Brief. Vol. 29 |
| · Dwivany, F.M., Nugrahapraja, H., Fukusaki, E., Putri, S.P., Novianti, C., Radjasa, S.K., Fauziah, T., Nirmala Sari, L.D. 2020. Dataset of Cavendish banana transcriptome in response to chitosan coating application. Data in Brief. Vol. 29 |
| · Abduh, M.Y., Nababan, E., Ginting, F., Juliati, J., dan Nugrahapraja, H. 2020. Biodelignification of lemon peels using Aspergillus to improve yield and composition of extxracted lemon oil. IIUM Engineering Journal. Vol. 21(2) |
| · Budiman, B.A., Purnama, H., Nugrahapraja, H., dan Zainuddin, I.M. et.al. 2019. Development of Appropriate Technology Adoption for Coffee Farmers in Sukawangi Village, Sumedang. Jurnal Ilmu Sosial, Politik dan Humaniora. P-ISSN 2654-3583. E-ISSN 2654-33382(2): 15-25 |
| · Esyanti, R.R., Dwivany, F.M., Mahani, S., Nugrahapraja, H., dan Meitha, K. Foliar application of chitosan enhances growth and modulates expression of defense genes in chilli pepper (Capsicum annuum L.). Australian Journal of Crop Science. E-ISSN 1835-2707. Vol.13(1): 55-60 |
| · Esyanti, R.R., Zaskia, H., Amalia, A., dan Nugrahapraja, H. 2019. Chitosan nanoparticle-based coating as post-harvest technology in banana. Journal of Physics: Conference Series. Vol.1204. Hal. 1-8Integrative annotation and characterisation of pectin methylesterase (PME) and pectin methylesterase inhibitors (PMEI) gene families in the Maize Genome |
Research and Development Projects
| · DYNAMIC MODULATION ON TRANSCRIPTIONAL NETWORK DURING CROSS-INCOMPATIBILITY OF Gametophyte factor1 (Ga1) LOCUS IN MAIZE |
| · Chitosan-Based Coating as an Alternative Post-harvest Technology for Banana |
| · Study on RNA Quality of Cavendish Banana (Musa acuminata AAA Group) Flesh Tissues with Different Preservation Methods Related to Expression of MaGAPDH, MaACS1, and MaACO1 Genes |
| · Application of Elongation Factor Genes in Agriculture: From Genomics to Metabolic Engineering. |
| · Growth Performance of Duckweed Fern Azolla pinnata R. Br. (Catfish eye) on Variation of Culture Medium. |
| · Developing Ecotourism as An Effort in Enhancing Competitiveness of Indonesia Tourism (Case Study: Ir. H. Djuanda Forest Park, Bandung) |
| · Modelling of Indonesia Tourism Marketing by Aligning Tourist’s Perception And Stakeholders’ Participation |
funding
| Year | Research Title |
| 2020 | Pengembangan Alat Deteksi COVID-19 Berbasis Data Genomik SARS-Cov2 Menggunakan Pendekatan Reverse Vaccinology |
| 2020 | Transfer Teknologi Pengembangan Kopi Spesialiti Citarum Harum 0 Km Menggunakan Rekayasa Proses Terkontrol (Proposal Lanjutan) |
| 2021 | Pendampingan Pemanfaatan Media Marketplace Sebagai Sarana Promosi Bumdes Untuk Pengembangan Desa Agrowisata Guranteng Pada Kelompok Informasi Masyarakat (KIM) |
| 2021 | Pengembangan Peptida 2.0: Dual Mode Action (Kandidat Vaksin dan Diagnostik Nano Molekuler) untuk HIV berbasis Kajian Reverse Vaccinology |
| 2021 | PPKI – Efek Paparan Mikroplastik Terhadap Komposisi Mikrobioma Usus dan Kesehatan Pencernaan pada Populasi Penduduk Pesisir di Jawa Timur |
| 2022 | Aktivitas Antivirus Dari Eksosom Sel Punca Manusia Terhadap Sars-Cov-2 Yang Menginfeksi Sel Vero |
| 2022 | Analisis Ekspresi Gen Menggunakan Pendekatan Transkriptomik Pada Induksi Ketahanan Tanaman Pisang Menggunakan Pupuk Silika Dan Kitosan |
| 2022 | Analisis Transkriptomik Pada Jalur Biosintesis Steviol Glikosida Yang Dielisitasi Jasmonat Dan Kaitannya Dengan Pertumbuhan Stevia Rebaudiana |
| 2022 | Fabrikasi Biosensor Bakteri secara Kolorimetri menggunakan Pendekatan Mikrofluida |
| 2022 | Identifikasi Dan Karakterisasi Senyawa Metabolit Dari Tanaman Lokal Indonesia Untuk Kandidat Senyawa Antimikroba Dan Antivirus Menggunakan Pendekatan Bioinformatika |
| 2022 | INTERNATIONAL CONFERENCE ON TRANSLATIONAL OMICS AND BIOINFORMATICS |
| 2022 | Kajian Strategis Respon Cekaman Kekeringan padi (Oryza sativa L.) lokal Indonesia melalui Analisis Metagenomik Komunitas Mikrobia dan Profiling Metabolomik |
| 2022 | Transfer Teknologi Pengembangan Kopi Spesialti Citanduy 0 Km menggunakan Rekayasa Proses Terkontrol |
| 2023 | Fabrikasi Biosensor Virus secara Kolorimetri menggunakan Pendekatan Mikrofluida |
| 2023 | Molecular Phylogenetic Analysis and DNA Barcode (PM) |
| 2023 | Response of Microbial Community Structure and Dynamics from Various Local Rice Varieties in Indonesia treated by Drought Stress using Omics Technology Approac |
| 2023 | BIOPROSPEKSI LAUT INDONESIA MELALUI GENOME MININGBIOPROSPEKSI LAUT INDONESIA MELALUI GENOME MINING |
| 2023 | Studi Biodiversitas Molekuler Mikroba Danau Halofilik Gili Meno Lombok |
| 2023 | Analisis Transkriptom Jaringan Tanaman Penghasil Gaharu Pascainokulasi Fusarium solani |
| 2024 | Pengembangan Marka eDNA (environmental DNA) mengunakan Teknologi Next-Generation Sequencing (NGS) untuk Pemetaan Biodiversitas Kawasan Pesisir Pantai dan Laut Utara Jawa Barat |
| 2019-2020 | Response of Microbial Community Structure and Dynamics of Chitosan Treated Banana during Fruit Ripening using Omics Technology Approach |
| 2021-2022 | Response of Microbial Community Structure and Dynamics of Black Soldier Fly Organic Waste Treatment using Omics Technology Approach |
| 2022-2025 | Pelatihan dan Pemeriksaan Sampel Spesimen |
| 2023-2024 | Response of Microbial Community Structure and Dynamics from Various Local Rice Varieties in Indonesia treated by Drought Stress using Omics Technology Approach |
| 2024 | MODULASI EPIGENETIK PADA REGENERASI SIRIP KAUDAL ZEBRAFISH TERPAPAR EKSTRAK PROPOLIS DAN NANOPARTIKEL ZNO HASIL GREEN SYNTHESIS |
| 2024 | PROFIL TRANSKRIPTOMIK SEL PUNCA KANKER OVARIUM PENYEBAB RESISTENSI KANKER OVARIUM |
| 2024 | Pengembangan kultivasi Spirogyra sp. dalam bioreaktor yang diberi cekaman salinitas untuk produksi asam lemak omega-3 dan penelaahan ekspresi gen-gen terkait |
| 2024 | Pemanfaatan Bioflavonoid Singkong sebagai Terapi Komplementer dalam Pencegahan Sindrom Metabolik : Kajian Molekuler pada Model In Vitro dan In Vivo |
| · NANOSCIENCES AND NANOTECHNOLOGY RESEARCH CENTER |
| · BIOSCIENCES AND BIOTECHNOLOGY RESEARCH CENTER – Secretary |
| · FORUM PENELITI MUDA INDONESIA (FORMIND) – HEAD |
| · THE INDONESIAN SOCIETY FOR BIOCHEMISTRY AND MOL. BIOLOGY |
| · THE INDONESIAN SOCIETY FOR BIOINFORMATICS AND BIODIVERSITY |
| · THE INDONESIAN BREEDING SOCIETY (PERIPI) |
Undergraduate Courses
- Introduction of Bioniformatics for Microbiology; Introduction of Bioinformatics for Biology;
- Metagenomics; Cellular and Molecular Biology; Cellular Biology; Internship; Synthetic Biology;
- Molecular Biology; Introduction to Scientific Techniques; Introduction to Computation.
Postgraduate Courses
- Master’s Research and Seminar; Bioinformatics; Molecular Ecology; Advanced Molecular Genetics
| Year | Courses |
| 2024/2025 | Pengantar Bioinformatika untuk Biologi |
| 2024/2025 | Praktikum Bioinformatika untuk Biologi |
| 2024/2025 | Pengantar Bioinformatika untuk Biologi |
| 2024/2025 | Praktikum Bioinformatika untuk Biologi |
| 2024/2025 | Berpikir komputasional |
| 2024/2025 | Bioinformatika |
| 2024/2025 | Praktikum Bioinformatika |
| 2024/2025 | Tren dalam Bioteknologi I |
| 2024/2025 | Rekayasa Metabolik berbasis Rekombinan |
| 2024/2025 | Data Sains untuk Genomik |
| 2024/2025 | Praktikum Data Sains untuk Genomik |
| 2024/2025 | Genetika Akuakultur |
| 2024/2025 | Metagenomik |
| 2024/2025 | Biologi Sistem |
| 2024/2025 | Konsep Biologi A |
| As the Principal Investigator |
| · DECIPHERING THE TRANSCRIPTOMICS LANDSCAPE OF MDCK AND SI6 CELL LINES THROUGH NEXT-GENERATION SEQUENCING |
| · Cooperation Grant from the For MIND Institute and PT BIO FARMA |
| As a Research Member |
| · The Development of Postharvest Management System by Utilizing Nanomaterial TiO2 with Banana Fruit as Model |
| · Utilization of Eco-Friendly Biochemistry (Nanocitosan) as Natural Fungicide Against Phytophthora capsici on Eight Cultivars of Chili Plants in West Java for Agroindustry Applications |
| · Transcriptome and Expression Profile Analysis of Banana Gene that Plays a Role in Ethylene Synthesis for Application in Agriculture Field |
| · The Use of Biomaterials Nano-chitosan as Biofungicide Against Phytophthora capsici on chillies cultivars in West Java Indonesia for sustainable agro-industries applications |
| · Fruit Storage Chamber as an alternative to post-harvest fruit storage |
| · The Application of Omics Technology in Banana Fruit Processing as a Standard of Food Industry Export |
| · GENES EXPRESSION STUDIES RELATED TO ETHYLENE BIOSYNTHESIS DURING BANANA RIPENING |
| · Establishment the Database of Biodiversity of Multiomics and Biogeography of the Exotic Banana of Maluku Islands Stick for Indonesian Food Security |
| · Development of Biogeography and Biodiversity Database for Smart Village Modeling Based on the Local Banana Commodities to Strengthen Sustainability of Village Economic Activity |
| · BPPT – National Task Force COVID-19 for Non-PCR Diagnostic. Member of Sub-Task Force for Peptides and Aptamer Design |
| · BPPT – National Task Force COVID-19 for Whole Genome Sequencing. Member of Sub-Task Force for Bioinformatics Analysis of SARS-CoV2 genome |
| · BSL-2 Advisor for Kota Bandung. COVID-19 Testing Laboratory Setup and Analysis |
| Year | Achievement | Activity | Organization | Location | Category: National/International |
| 2023 | Visiting fellow/professor di Perguruan Tinggi Luar Negeri | Kolaborasi riset | Universiti Malaysia Terengganu | Malaysia | Internasional |
| 2023 | Visiting fellow/professor di Perguruan Tinggi Luar Negeri | Pembicara Tamu | Global Engage | Thailand | Internasional |
English 
Indonesian