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KARLIA MEITHA, Ph.D.

KARLIA MEITHA, Ph.d.

Assistant Professor
Genetics and Molecular Biotechnology
School of Sciences and Technology
Bandung Institut of Technology
Email : karliameitha@itb.ac.id
SINTA | Google Scholar | Scopus

OVERVIEW

Karlia Meitha, Ph.D. primarily focuses in Genetics an Molecular Biotechnology, specifically in the research of genome editing; small-RNAs for biocontrol; epigenetics; and high-antioxidant production in crops

Bachelor’s Degree

  • Bandung Institute of Technology, Indonesia (2006).

Master’s Degree

  • Bandung Institute of Technology, Indonesia (2010).

Doctoral Degree

  • Universiy of Western Australia, Australia (2017).
Year Institution Position
Dec 2017 – 2020 The Research Foundation of Indonesia Biogeography and Biodiversity Director, Bandung, Indonesia
Feb 2017 – 2020 Bali International Research Centre for Banana Secretary Bali, Indonesia
Jan – Dec 2015 Jan – Dec 2014 The Association of Indonesian Postgraduate Students and Scholars in Australia Vice President Treasurer Perth, Australia
Jan 2018 – Present Institut Teknologi Bandung Bandung, Indonesia Jan 2018 – Present
Oct 2016 – Dec 2017 Institut Teknologi Bandung Bandung, Indonesia Academic Assistant and Postdoctoral Fellow
Aug – Nov 2014 The University of Western Australia Demonstrator for laboratory practice
Jun 2013 – Sep 2013 Leeds University Leeds, UK Visiting researcher
  1. Wicaksono, A., Meitha, K., Wan, K., Isa, M. N. M., Parikesit, A. A., & Molina, J. (2025). Hairpin in a haystack: In silico identification and characterization of plant-conserved microRNA in Rafflesiaceae. Open Life Sciences, 20(1). https://doi.org/10.1515/biol-2022-1033
  2. Septiani, P., Pramesti, Y., Ghildan, M. et al. RNAi-based biocontrol for crops: a revised expectation for a non-recent technology. Planta 261, 44 (2025). https://doi.org/10.1007/s00425-025-04625-0
  3. Hermawaty, D., Putri, M. A., Prabhandiya, S., Dwivany, F. M., & Meitha, K. (2025). Epigenetic regulation of banana fruit ripening: global and specific DNA methylation profile, and characterization of DNA methylation-related genes. Cogent Food & Agriculture, 11(1). https://doi.org/10.1080/23311932.2024.2448267
  4. Fauziah, T., Esyanti, R. R., Meitha, K., Iriawati, N., Hermawaty, D., & Wijayanti, G. a. I. F. (2024). Cell cycle arrest via DNA damage response (DDR) pathway induced by extracellular Self-DNA (ESDNA) application in rice root. Plant Physiology and Biochemistry, 219, 109370. https://doi.org/10.1016/j.plaphy.2024.109370
  5. Dwivany, F. M., Fauziah, T., Yamamoto, K., Novianti, C., Perwira, K. P. C., Rizanti, M., Radjasa, S. K., Hakim, F. S., Salim, A. S. P., Putri, R. R., Wicaksono, A., Sumardi, D., Putri, S. P., Fukusaki, E., Meitha, K., & Nugrahapraja, H. (2024). Chitosan coating to delay the ripening process in banana: a transcriptomics study. Horticulture Environment and Biotechnology, 66(1), 123–136. https://doi.org/10.1007/s13580-024-00642-2
  6. Manuhara, Y. S. W., Sugiarso, D., Fajar, A. P., Niam, K., Aziz, R. T., Yudha, A. W., Clement, C., Daryono, B. S., Miftahudin, M., Meitha, K., Nurhayati, A. P. D., & Wibowo, A. T. (2024). Optimizing Growth of Melon (Cucumis melo L. cv. Madesta) in Nutrient Film Technique and Drip Irrigation Hydroponics with Varied Substrates. Pertanika Journal of Tropical Agricultural Science, 47(4), 1191–1204. https://doi.org/10.47836/pjtas.47.4.09
  7. Negara, H. a. S. M. P., Esyanti, R. R., Iriawati, I., Signorelli, S., Kirana, R., & Meitha, K. (2024). Enhancing genetic modification in recalcitrant plants: An investigation in chili (<i>Capsicum annuum</i>) through the optimized tape sandwich protoplast isolation and polyethylene glycol-mediated transfection. Plant Biotechnology, 41(4), 459–464. https://doi.org/10.5511/plantbiotechnology.24.0613a
  8. Meitha, K., Chen, P. Y., Chang, C., Lin, Z. C., Hsu, T. W., Gojobori, T., & Chiang, T. Y. (2024). Is an invasive species a notorious invader or carbon sequencer?. Ecological Genetics and Genomics, 100279.
  9. Hermawaty, D., Meitha, K., & Esyanti, R. R. (2024). The Potential of Methylation Sensitive Amplification Polymorphism (MSAP) Technique to Study Global DNA Methylation Changes in Plants: Basic Principles and Perspective. Reviews in Agricultural Science, 12, 93-110.
  10. Septiani, P., Pramesti, Y., Ningsih, D. U., Pancaningtyas, S., & Meitha, K. (2024). Identification of self-and pathogen-targeted miRNAs from resistant and susceptible Theobroma cacao variety to black pod disease. Scientific Reports, 14(1), 3272.
  11. Tham, J., Patisenah, A., Tjia, T. O. S., Signorelli, S., Taufik, I., & Meitha, K. (2023). sgRNA design and in vitro nucleolytic analysis of the Cas9‐RNP complex for transgene‐free genome editing of the eIF4E1 gene from Capsicum an‐nuum L. Indonesian Journal of Biotechnology, 28(4), 238-247.
  12. Meitha, K., Hanisia, R. H., Signorelli, S., Fauziah, T., Iriawati, and Esyanti, R. R. (2023) Extracellular DNA of Fusarium oxysporum sp. cubense as a priming agent for inducing the resistance of banana plantlets. Agronomy. 13(2:441). https://doi.org/10.3390/agronomy13020441
  13. Wiprayoga, I., Meitha, K., and Dwivany, F.M. (2023) In Silico Characterization of Lycopene Beta Cyclase (LCYB) and Lycopene Epsilon Cyclase (LCYE) Genes from DH-Pahang (Musa acuminata, A Genome) and DH-PKW (Musa balbisiana, B Genome). Journal of Tropical Lofe Sciences. 13(1). https://doi.org/10.11594/jtls.13.01.09
  14. Tjia, T. O. S., Meitha, K., Septiani, P., Awaludin, R., and Sumardi, D. (2023) Extracellular self-DNA induces local inhibition of growth, regulates production of reactive oxygen species, and gene expression in rice roots. 67, 9-18. DOI: 10.32615/bp.2022.037
  15. Meitha, K., Pramesti, Y., Signorelli, S., and Kriswantoro, J.A. (2022) Postharvest chitosan application maintains the quality of spinach through suppression of bacterial growth and elicitation. Horticulture, Environment, and Biotechnology. 63(2), 217-227. https://doi.org/10.1007/s13580-021-00397-0
  16. Suhandono, S., Kusuma, S. H., Meitha, K. (2021) Characterization and Production of Rhamnolipid Biosurfactant in Recombinant Escherichia coli Using Autoinduction Medium and Palm Oil Mill Effluent. Brazilian Archives of Biology and Technology. https://doi.org/10.1590/1678-4324-2021200301
  17. Meitha, K., Esyanti, R. R., and Hanisia, R. H. (2021) Green pesticide: Tapping to the promising roles of plant secreted small RNAs and responses towards extracellular DNA. Non-coding RNA Research. 6(1). 42-50. https://doi.org/10.1016/j.ncrna.2021.02.001
  18. Setiabudi, E., Meitha, K., and Dwivany, F. M. (2021) In silico characterization and comparison of the fruit ripening related beta‐amylase (BAM) gene family in banana genome A and B. Indonesian Journal of Biotechnology. 26(4). https://doi.org/10.22146/ijbiotech.65142
  19. Kusuma, S. H., Meitha, K., Suhandono, S. (2021) Characterization of Di-Rhamnolipid Biosurfactant in Recombinant Escherichia coli. Key Engineering Materials.https://doi.org/10.4028/www.scientific.net/KEM.874.107
  20. Dwivany, F. M., Sukriandi, N., Meitha, K., Brotosudarmo, T. H. P. (2021) In silico Characterization of the Structure of Genes and Proteins related to β-carotene Degradation in Musa acuminata ‘DHPahang’ and Musa balbisiana’Pisang Klutuk Wulung’. Pertanika Journal of Tropical Agricultural Science. 44(2). DOI: https://doi.org/10.47836/pjtas.44.2.10
  21. Nugrahapraja, H. N., Dwivany, F. M., Meitha, K., Kuswati, Esyanti, R. R. (2021) Chitosan improving Growth in Chili (Capsicum annuum L.) Plants and acting through Distinct Gene Regulation between Cultivars. Research Journal of Biotechnology. 16(2)
  22. K. Pramesti, Y., Suhandono, S. (2020) Reactive oxygen species and antioxidants in postharvest vegetables and fruits. International journal of food science. https://doi.org/10.1155/2020/8817778
  23. Meitha, K., Fatmawati, I., Dwivany, F.M., Sutanto, A., Pratama, S.N., Nugrahapraja, H., and Wikantika, K. (2020) Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.49506
  24. Aziz, M.A., Esyanti, R.R., Meitha, K., Dwivany, F.M., and Chotimah, H.H. (2020) Chitosan suppresses the expression level of WRKY17 on red chili (Capsicum annuum) plant under drought stress. Indonesian Journal of Biotechnology. 25(1). doi: 10.22146/ijbiotech.55016
  25. Dwivany, F. M., Ramadhan, M. R., Lim, C., Sutanto, A., Nugrahapraja, H., Wikantika, K., Pratama, S. N., Meitha, K., Pratiwi, A. S. (2020). Bali Bananas (Musa spp. L.) Genetic Relationship Based on Internal Transcribed Spacer 2 (ITS-2). Pertanika Journal of Tropical Agricultural Science. 43(4)
  26. Esyanti, R.E., Dwivany, F. M., Mahani, S., Nugrahapraja, H. & Meitha, K. (2019) Foliar application of chitosan enhances growth and modulates expression of defense genes in chilli pepper (Capsicum annuum L.). Australian Journal of Crop Sciences. 13(01). doi: 10.21475/ajcs.19.13.01.p1169
  27. Meitha, K., Agudelo-Romero, P., Signorelli, S., Considine, J., Foyer, C. & Considine, M. (2018) Developmental Control of Hypoxia During Bud Burst in Grapevine. Plant, Cell, and Environment. doi:10.1111/pce.13141
  28. Signorelli, S., Agudelo-Romero, P., Meitha, K., Foyer, C. H., & Considine, M. J. (2018) Roles for Light, Energy, and Oxygen in the Fate of Quiescent Axillary Buds. Plant Physiology. doi: 10.1104/pp.17.01479
  29. Rai, I N., Dwivany, F. M., Sutanto, A., Meitha, K., Sukewijaya, I M., and Ustriyana, I N. G. (2018) Biodiversity of Bali Banana (Musaceae) and its Usefulness. Hayati Journal of Biosciences. 25 (2). doi: 10.4308/hjb.25.2.47
  30. Hidayat, T., Kelana, H.W., Ismanto, D.I.A., and Meitha, K. (2018) Survey on Ethnobotanic Value of Banana (Musa spp; Musaceae) in Bali Province, Indonesia. Hayati Journal of Biosciences. 25 (1). doi:10.4308/hjb/25.1.31
  31. Shaw, D. S., Meitha, K., Considine, M. J., & Foyer, C. H. (2017). Mitochondrial Respiration and Oxygen Tension. In K. Jagadis Gupta (Ed.), Plant Respiration and Internal Oxygen: Methods and Protocols (pp. 97-113). New York, NY: Springer New York. https://doi.org/10.1007/978-1-4939-7292-0_11
  32. Meitha, K. & Considine, M. (2017) Regulation of Oxygen and Reactive Oxygen Species During Budburst. Wine and Viticulture Journal. 32:4. (pp. 42-45)
  33. Meitha, K. (2016). Promotor Gen: Tombol On/Off Ekspresi Gen yang Penting Bagi Kelangsungan Hidup Organisme. In Ketut Wikantika (Ed.), Bunga Rampai Forum Peneliti Muda Indonesia (pp. 9-14). Bandung: Penerbit ITB. ISBN: 978-602-7861-52-7
  34. Meitha, K., Konnerup, D., Colmer, T. D., Considine, J. A., Foyer, C. H., & Considine, M. J. (2015). Spatio-temporal relief from hypoxia and production of reactive oxygen species during bud burst in grapevine (Vitis vinifera). Annals of Botany (Reactive Oxygen and Nitrogen Species). doi:10.1093/aob/mcv123

HAKI

  1. SEDIAAN MEDIA TRANSPORT VIRUS INAKTIF UNTUK MENYIMPAN VIRUS TERINAKTIVASI SECARA AMAN DAN METODE PEMBUATANNYA Nomor Permohonan : P00202214780
  2. PLASMID REKOMBINAN DAN SEL INANG Escherichia coli REKOMBINAN PENGHASIL DIRHAMNOLIPID SERTA METODE PRODUKSI DIRHAMNOLIPID DENGAN KOMPOSISI MEDIA EKSPRESI YANG OPTIMUM MENGGUNAKAN PLASMID DAN SEL INANG TERSEBUT DALAM MENGHASILKAN VARIAN DIRHAMNOLIPID SEBAGAI BIOSURFAKTAN Nomor Permohonan : P00202001556
Year Activity Leader Personnel
2023 MOLECULAR PHYLOGENETIC ANALYSIS AND DNA BARCODE [SITH.PPMI-2-07-2023] Dadang Sumardi  Fenny Martha Dwivany; Adi Pancoro; Sony Suhandono; Husna Nugrahapraja; Azzania Fibriani; Ernawati Arifin Giri-rachman; Karlia Meitha; Popi Septiani;
2024 MENGGALI POTENSI SUMBERDAYA GENETIK PADI LOKAL MELALUI PEMULIAAN PARTISIPATIF DI KECAMATAN TANJUNGSARI KABUPATEN SUMEDANG [SITH.PPMI-2-11-2024] Popi Septiani Dadang Sumardi; Husna Nugrahapraja; Azzania Fibriani; Ernawati Arifin Giri-rachman; Sony Suhandono; Adi Pancoro; Karlia Meitha; Fenny Martha Dwivany;

funding

Year Research Title
2020 Pengadaan Virus Transport Media (VTM) Untuk Mendukung Pengujian Terhadap Virus SARS-Cov2
2020 Rekayasa Kultivasi Microgreens dalam Sistem Urban Farming Untuk Meningkatkan Kandungan Antioksidan
2021 Optimasi Pembuatan Virus Transport Media (VTM)-Inactivated Sebagai Produk Kemandirian Bangsa yang Mampu Berkontribusi pada Penanganan Penyebaran Covid-19
2021 Pengembangan Cabai (Capsicum annuum)  Tahan Hama Potyvirus melalui Modifikasi Gen eIF4E menggunakan Metode Edit Genom CRISPR-Cas9
2022 Analisis Mekanisme “Cell Cycle Arrest” Akibat Perlakuan Self- Dan Non Self Extracelullar Dna” Pada Kecambah Padi
2022 Identifikasi MiRNA Theobroma Cacao Sebagai Kandidat Biopestisida Phytophthora Palmivora
2022 Pengembangan Sistem Sintesis Protein Cas9 dengan Solubilitas Tinggi untuk Edit Genom Berbasis CRISPR-Cas9 BebasTransgenik
2023 Analisis Global Metilasi DNA untuk Pengembangan Manajemen Pascapanen
2023 Analisis Transkriptomik dan Metabolomik dalam Biosintesis Karotenoid dan Toleransi Cekaman Abiotik pada Genotipe Melon Indonesia Hasil Aplikasi Potassium
2023 Edit Genom Daerah Cetakan miRNA di Cabai untuk Meningkatkan Ketahanan terhadap Phytophthora capsica
2023 International Student Conference (ISC)
2023 Molecular Phylogenetic Analysis and DNA Barcode (PM)
2023 Studi Biodiversitas Molekuler Mikroba Danau Halofilik Gili Meno Lombok
2024 STUDI BIODIVERSITAS DANAU GILI MENO – LOMBOK DENGAN
PENDEKATAN GENOMIK
2024 Potensi Carbon-Nanodots sebagai komponen bahan lokal untuk mensubtitusi koloid emas pada tes diagnostik SARS-COV-2
2024 PRODUKSI PROTEIN SPIDROIN REKOMBINAN UNTUK REKAYASA
JARINGAN KARTILAGO [SITH.PPMI-1-11-2024]
2024 SKRINING KETAHANAN TERHADAP CEKAMAN KEKERINGAN PADA PADI
LOKAL

Professional membership

  • International Society for Horticultural Science, 2022 – present
  • Asian Federation of Biotechnology (Indonesia Chapter), 2022 – present
  • Indonesian Biotechnology Program Association, 2020 – present
  • Society of Experimental Biology, London, United Kingdom, 2013 – present

Undergraduate Courses

  • Genetics; Syntetic Biology; Fundamentals for Protein Engineering;
  • Cellular and Molecular Biology; Microbial Genetics and Genetic Engineering

Postgraduate Courses

  • Advanced Molecular Genetics; Biotechnology of Postharvest; Plant Stress and Biotechnology
  • Research Design, Bioethics, and Patent; Biotechnology Research and Seminar; Thesis and Final Defense
Year Courses
2024/2025 Praktikum Genetika
2024/2025 Biologi Molekuler
2024/2025 Biologi Molekuler
2024/2025 Genetika dan Biologi Molekuler
2024/2025 Praktikum Genetika dan Biologi Molekuler
2024/2025 Penulisan dan seminar proposal
2024/2025 Penelitian Magister I
2024/2025 Penelitian Magister II dan Seminar A
2024/2025 Sidang Magister
2024/2025 Bioteknologi Ketahanan Tumbuhan
2024/2025 Tren dalam Bioteknologi I
2024/2025 Metodologi Penelitian
2024/2025 Dasar Rekayasa Protein
2024/2025 Proyek Biologi Sel Molekuler dan Genetika Mikroba
2024/2025 Genetika dan Biologi Molekuler
2024/2025 Praktikum Genetika dan Biologi Molekuler
2024/2025 Tren dalam Bioteknologi I
2024/2025 Metodologi Penelitian
2024/2025 Penulisan dan seminar proposal
2024/2025 Penelitian Magister I
2024/2025 Nanoteknologi untuk Bioteknologi
2024/2025 Penelitian Magister II dan Seminar A
2024/2025 Penulisan Tesis
2024/2025 Sidang Magister

Awards

  • 2023 National Fellowship L’Oreal UNESCO for Women in Science

L’Oreal Unesco for Women in Science

2023
  • Keynote Speaker

Joint Seminar and Focus Group Discussion “Toward Sustainable Agriculture: The Role of Plant Breeding and Biotechnology”

2023
  • Visiting fellow/professor di Perguruan Tinggi Luar Negeri

Inisiasi kolaborasi pendidikan dan riset

2023
  • The Matsumae International Fellowship

Research visit to Dr. Keiji Nishida laboratory at Kobe University, Japan

Nov 2022 – Feb 2023

 

  • Award in Teaching Excellence

Granted by the Rector of ITB at the 63rd Dies Natalis

Feb 2022
  • The Best Paper Presenter

The 5th International Biology Conference, Biology Department, ITS – Indonesia

October 2020
  • Travel Grant from Society for Experimental Biology

To attend annual main meeting in Prague, Czech.

Jun – Jul 2015
  • Travel Grant from Grape and Wine Research Centre Australia

Research visit to Prof. Christine Foyer laboratory at Leeds University.

Jun – Sep 2013
  • PhD Scholarship from Australia Awards

The University of Western Australia.

Jun 2012 – Jul 2016
  • Best Oral Presenter at International Seminar “Biotechnology for A Sustainable Future”

Udayana University, Bali – Indonesia.

 Sep 2009

 

Grants

1.      Riset Kolaboratif antar KK- ITB

Edit Genome of MiRNA Template Regions in Chili to Increase Resistance to Phytophthora capsici

Mar – Nov 2023

 

2.      Riset Peningkatan Kapasitas KK – ITB

Global Analysis of DNA Methylation for Postharvest Management Development

Mar – Nov 2023

 

3.      Riset Unggulan – ITB

Development of Highly Soluble Cas9 Synthesis System for Transgenic Free CRISPR-Cas9 Genome Engineering

Feb – Nov 2022

 

4.      PPMI KK GBM – SITH ITB

Identification of Theobroma cacao miRNA as Biopesticide Candidate for Phytophthora palmivora

Feb – Nov 2022
5.      Riset Unggulan – ITB

Development of Potyvirus Resistant Chili (Capsicum annuum) through Modification of the eIF4E Gene using the CRISPR-Cas9 Genome Editing Method

Feb – Nov 2021
6.      Riset Inovasi LPiK – ITB

Optimization of the manufacture of Inactivated Virus Transport Media (VTM)

Feb – Nov 2021
7.      Research Grant (PT. Rekacipta Inovasi – ITB)

Recovery Rate Determination of Activated Virus Transport Medium

Feb – Nov 2021
8.      Research Grant (LPiK – ITB)

The preparation of viral transport media for local detetion center in Bandung

May – Oct 2020
9.      Riset peningkatan kapasitas – ITB

Improving antioxidant contents in microgreens by combination of red and blue light

10.  Research Grant P3MI – ITB

Effect of Chitosan Coating on the Postharvest Quality and Regulation of Gene Expression Related to ROS (Reactive Oxygen Species) od Spinach (Spinacia oleracea)

Feb – Nov 2020

 

 

Feb – Nov 2019

The Research Foundation of Indonesia Biogeography and Biodiversity Director, Bandung, Indonesia
Dec 2017 – 2020
Plan, lead, and review organisational development strategies with the board of directors
Bali International Research Centre for Banana
SecretaryBali, Indonesia
Feb 2017 – 2020
Facilitate the communication between two main universities involved in the research, ITB and Udayana University; Managing research activities.
The Association of Indonesian Postgraduate Students and Scholars in Australia Vice President
Treasurer Perth, Australia
Jan – Dec 2015
Jan – Dec 2014
Collaborated with other community organizations in Perth to create friendship events in sports and
women in leadership.Promoted and managed fund-raising projects.Informed future Indonesian students about education systems and life in Australia.
Bandung Institut of Technology
Lecturer and Researcher
Bandung, Indonesia
Jan 2018 – Present
· Facilitate students to grasp the knowledge in Biology topics and related research skills

· Conduct research in the field of plant biomolecular and its application

Institut Teknologi Bandung Bandung, Indonesia
Academic Assistant and Postdoctoral Fellow Oct 2016 – Dec 2017
· Conducted biomolecular research in the topics of banana diversity.

· Assisted the curriculum evaluation process and preparation of new curriculum documents for Microbiology
and Biotechnology study programs.

The University of Western Australia
Demonstrator for laboratory practice
Perth, Australia
Aug – Nov 2014
· Guided undergraduate students to perform essential physiology and molecular experiments.

· Helped the students to understand crucial concepts underlying the experiments.

Leeds University Leeds, UK
Visiting researcher Jun 2013 – Sep 2013
· Observed the localization of reactive oxygen species in grape bud tissues by using histological method.

· Examined techniques for mitochondria extraction and oxygen consumption rate measurements.

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