Pramesti Istiandari, M.Eng, Ph.D.
Lecturer
Plant Sciences and Biotechnology
School of Sciences and Technology
Bandung Institut of Technology
Email : pramesti@itb.ac.id
SINTA | Google Scholar | Scopus
Pramesti Istiandari, M.Eng., Ph.D., specializes in plant biotechnology, focusing on the biosynthesis of triterpenoids in legumes such as Lotus japonicus and Abrus precatorius. Her research employs genomic and transcriptomic studies, metabolite profiling, and genetic engineering using CRISPR/Cas9. Her research emphasizes profiling specialized plant compounds using GC-MS, particularly terpenoid compounds. She also explores metabolic engineering in transgenic Saccharomyces cerevisiae for heterologous triterpenoid production. Her work aims to deepen the understanding of secondary metabolite pathways and advance biotechnological applications for plant-based products.
| Prsentasi | |
| 2020 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2020. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. Academic Exchange Seminar Between Shanghai Jiao Tong University and Osaka University, 2nd Industrial Biotechnology Symposium (Online), Japan. |
| 2021 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2021. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. The 62nd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| 2022 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2022. Comparative Analysis of NADPH-Cytochrome P450 Reductase Classes of Lotus japonicus Towards Triterpenoids Biosynthesis. The 63rd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| 2019 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 女子大学院生と企業等との交流会. Osaka, Japan. (Poster Presentation). |
| 2019 | Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 37th Annual Meeting of the Japanese Society for Plant Cell and Molecular Biology. Kyoto, Japan. |
| Journals & Proceedings |
| Istiandari, P., Yasumoto, S., Seki, H., Fukushima, E. O., & Muranaka, T. (2023). Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus. Frontiers in Plant Science, 14, 1214602. |
| Istiandari, P., Yasumoto, S., Srisawat, P., Tamura, K., Chikugo, A., Suzuki, H., et al. (2021). Comparative analysis of NADPH-cytochrome P450 reductases from legumes for heterologous production of triterpenoids in transgenic Saccharomyces cerevisiae. Frontiers in Plant Science, 12, 762546. |
| Abduh, M. Y., Jamilah, M., Istiandari, P., Manurung, R., & Manurung, R. (2017). Bioconversion of rubber seeds to produce protein and oil-rich biomass using black soldier fly larva assisted by microbes. Journal of Entomology and Zoology Studies, 5(4), 591-597. |
| Presentations |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 37th Annual Meeting of the Japanese Society for Plant Cell and Molecular Biology. Kyoto, Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2020. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. Academic Exchange Seminar Between Shanghai Jiao Tong University and Osaka University, 2nd Industrial Biotechnology Symposium (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2021. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductase Classes of Legumes Towards Triterpenoids Biosynthesis. The 62nd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2022. Comparative Analysis of NADPH-Cytochrome P450 Reductase Classes of Lotus japonicus Towards Triterpenoids Biosynthesis. The 63rd Annual Meeting of the Japanese Society of Plant Physiologists (Online), Japan. |
| Istiandari P., Yasumoto S., Seki H., Fukushima E.O., Muranaka T. 2019. Comparative Analysis of Plant NADPH-Cytochrome P450 Reductases Towards Heterologous Triterpenoid Production in Transgenic Yeast. 女子大学院生と企業等との交流会. Osaka, Japan. (Poster Presentation). |
| Year | Activity | Leader | Personnel |
| 2024 | EQUIPPING HIGH SCHOOL STUDENTS WITH BIOENTREPRENEURIAL SKILLS THROUGH A YOGURT FERMENTATION WORKSHOP | Ihsan Tria Pramanda | Putu Virgina Partha Devanthi; Mario Donald Bani; Dina Hermawaty; Pramesti Istiandari; Wildan Qoharisma Salam; Riahna Kembaren; |
PROYEK PENELITIAN
| Year | Research Title |
| 2023 | TRANSCRIPTOMIC ANALYSIS OF MEDICINAL LEGUME ABRUS PRECATORIUS TREATED WITH METHYL JASMONATE TO REVEAL THE BIOSYNTHETIC GENES OF ABRUSOSIDE AS A POTENT NATURAL SWEETENER AND THERAPEUTIC AGENT |
| 2024 | ENHANCING THE PRODUCTION OF THERAPEUTIC TRITERPENOID SAPONINS IN SAGA (ABRUS PRECATORIUS) THROUGH HYDROPONIC CULTIVATION |
| 2024 | MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERIZATION OF PLANT GROWTH–PROMOTING MICROBES FROM COMPOST-TREATED SOIL AND PLANT |
| 2024 | Optimasi metode kultur jaringan tanaman sacha inchi (Plukenetia volubilis. L) yang berkelanjutan dan resilien terhadap perubahan iklim |
HIBAH PENELITAN
| Optimizing Tissue Culture Methods for the Propagation of Sacha Inchi (Plukenetia volubilis L.) as a Sustainable and Climate-Resilient Functional Food Source. Ministry of Research and Education. 2024. |
| Unveiling High-Value Triterpenoid Biosynthesis in Medicinal Legume Abrus precatorius Through Transcriptomic Analysis and Methyl Jasmonate Treatment. Toray Science and Technology Research Grant. 2023. |
| Enhancing the Production of Therapeutic Triterpenoid Saponins in Saga (Abrus precatorius) through Hydroponic Cultivation. Indonesia International Institute for Life Sciences Internal Grant. 2023. |
| Year | Courses |
| 2024/2025 | Neraca Massa dan Energi Sistem Hayati |
| 2024/2025 | Kapita Selekta Biologi |
| 2024/2025 | Pengantar Rekayasa Hayati |
| 2024/2025 | Rekayasa Bioproduk |
| 2024/2025 | Rekayasa Sel dan Jaringan Tanaman |
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