Cell suspension and callus cultures may serve as an alternative solution to provide a fast-growing plant material to produce artificial agarwood in a short period. Here, Dr. Ahmad Faizal, Dr. Rizkita Rachmi Esyanti, and their colleagues induced agarwood formation in callus cultures of Aquilaria malaccensis by application of crude mycelial extracts of Fusarium solani strains GSL1 or GSL2, or methyl jasmonate. The gas chromatography–mass spectrometry analysis identified 33 different secondary metabolites among all samples, four of which were present in all treatments and control, i.e., 1-docosene and 1-octadecene, 4-di-tert-buthylphenol, and benzenepropanoic acid. The 6-methoxy-2-(4-methoxyphenethyl)-4H-chromene-4-one, a chromone derivative, was only detected in callus elicited with the F. solani strain GSL2 and MeJA. All treated calli produced more fatty acid derivatives than the control group. They conclude that elicitors used in this study can induce the production of agarwood-related chemicals such as chromone and fatty acid in callus culture.

Article Citation:
Faizal, A., Esyanti, R. R., Utami, N. P., Azar, A. W. P., Hermawaty, D., Setiyobudi, T., Martin, A. F., Hapsari, B. W., Turjaman, M. (2023). Elicitation of Secondary Metabolites in Aquilaria malaccensis Lam. Callus Culture by Crude Mycelial Extract of Fusarium solani and Methyl Jasmonate. Forests; 14 (1), 48. https://doi.org/10.3390/f14010048

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Forests Journal cover
(Source: https://www.mdpi.com/journal/forests)
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Profile of Dr. Ahmad Faizal:
https://sith.itb.ac.id/en/dr-ahmad-faizal/

Profile of Dr. Rizkita Rachmi Esyanti:
https://sith.itb.ac.id/dr-rizkita-rachmi-esyanti/