Standard Operational Procedure (SOP) for the Request of Plant and Animal Identification and Determination at the School of Life Sciences and Technology ITB.

Steps in the Plant/Animal Identification and Determination

  1. Write a request letter to the Vice Dean of Resources SITH ITB, Labtek XI building,  1st Floor, Jalan Ganesha No. 10, Bandung 40132 from the institution of the applicant, signed by the appropriate officer.
  2. Pay the Identification/ Determination fee at Bank BNI:

Penampungan SITH-ITB, Account no : 0901062011

Identification and determination fee :

Rp. 55,000  (fifty five thousand rupiah) per plant sample,

Rp. 110,000  (one hundred and ten thousand rupiah) per animal sample

Specifically for Bachelor and Master student from SITH ITB, the identification fee is half of the normal fee.  The student should write a request for the identification/determination.  The letter should be signed by the student and co-signed by the student’s supervisor.

For private companies, the fee is twice the normal fee.

Payment can only done through the teller of the bank, with the following information provided:

  • Name of the student making the payment.
  • NPWP for the ones who already have one or personal identification number (No. KTP/ NIK)
  • In the message collumn write the type of plants/animals to be identified/determined and the student’s name and registration number (NIM/ NPM)
  1. The photocopy of the request letter is handed together with:
  2. Proof of payment from Bank BNI
  3. Plant/animal specimen to be identified.

Requirements for the conditions of the plant specimen to be identified:

  • Fresh plants or plants preserved in alcohol. Method of plant preservation to be used in determination:  The plant should be wrapped in newspaaper and tied with a plastic raffia string.  The wrapped plant is placed inside a transparent plastic bag and 70% alcohol is poured into the plastic bag.  The plastic bag is then sealed with duct tape.
  • For algae samples, the samples to be handed in have to be complete, that include the reproductive organs and the samples are placed inside an air tight container filled with 70% alcohol.
  • For moss samples, the samples to be handed in are the whole parts of the moss, including the reproductive organs.
  • For fern samples (pteridophytes), the samples to be handed in have to be complete, including the root, stem, sterile leaf without spores, fertile leaf with spores (Figure 1). Specifically for large ferns, bring a cut of the stem of the leaf and an intact sterile leaf and fertile leaf (Figure 2).
  • For herbaceous plant samples, the samples to be handed in have to be complete, including the root, stem, leaf, flower and fruit (Figure 3). If the fruit is not available, than the flower would be sufficient.  Specifically for large herbaceous plants such as red ginger (Etlingera elatior), tepus (Achasma megalacheilosor) or banana plant, bring the leaf, inflorescence and fruit (Figure 4).
  • For bushes, shrubs and tree samples, the samples to be handed in are in the form of a 30 cm long branch, complete with leaves, flowers and fruits (Figure 5). The samples should be in an intact condition, consisting of branch, leaves, flowers and fruits.  If the fruit is not available, the flower would be sufficient.  .
  • For bamboo samples, the samples to be handed in are a cut of the reed, midrib, leaf, and if possible the flower and fruit (Figure 6).

For rattan samples, the samples to be handed in are a cut of the stem, cut of the leaf stem, leaf, flagellum (usually located at the tip of the leaf), one inflorescence and fruit (Figure 7).

Figure 1.  Sample of ferns.  Parts that are handed in for determination are the roots, stem, sterile leaves and fertile leaves (Source of the picture: http://v3.boldsystems.org).

Figure 2.  Samples of large ferns.  Parts to be handed in are a cut og the leaf stem, complete sterile leaf and  complete fertile leaf  (Source of the picture: http://v3.boldsystems.org).

Figure 3.  Samples of small herbaceous plants.  Samples to be handed in for determination are the whole pant, complete with the root, stems, leaves, and reproductive organs (flowers and fruits)  (Source of the picture: http://portal.cybertaxonomy.org).

Figure 4. Samples of large herbaceous plants.  Samples to be handed in are the leaves, inflorescence and fruits (Source of the picture:: researchgate.net).

Figure 5. Samples of  bushes, shrubs and trees.  Samples to be handed in are a 30 cm long branch, complete with the leaves, flowers and fruits.

Figure 6. Samples of bamboo. Samples to be handed in are a cut of the reed, midrib, leaf, and if possible the reproductive organs (flowers and fruits) (Source of the picture:  researchgate.net).

Figure 7. Samples of rattan. Samples to be handed in are a cut of the stem, cut of the leaf stem, leaf, flagellum and if possible, the reproductive organs (flowers and fruits) (Source of the picture: researchgate.net).

Requirements for the conditions of the animal samples to be identified:

Intact specimens with all body parts complete, no damaged or lost parts, handed in alive, have the following requirements:

  1. Specimens are adults (except for insect larvae) with a maximal size of 1 meter.
  2. Specimens are brought in a suitable substrate
  3. Sea water: for animals originating from the sea such as ocean fishes, sea cucumbers, crabs, shrimps, brine shrimps (Artemia), jelly fishes, squids, starfishes, sea slugs and sea urchins.
  4. Fresh water:  for animals from fresh water such as freshwater fishes, eels, freshwater crabs, freshwater slugs, worms and water insects.
  5. Earth: for specimens that live underground such as earthworm and ground insects..
  6. Plant substrate in a container or cage for:  specimens living in certain plants or specimens that could be kept in plant subtrates such as insects, amphibians, reptiles, chickens, birds and small mammals.

Specimens that are intact and well preserved, have the following requirements:

  1. Specimens have been fixed with 10% Neutral Buffered Formalin, with the following composition: 100 ml Formalin (37-40% stock solution), 900 ml Water, 4 grams sodium dihydrogen phosphate, monohydrate (NaH2PO4.H2O) and 6.5 grams disodium hydrogen phosphate, anhydrous (Na2HPO4).  The recipe could be seen at  http://stainsfile.info/StainsFile/prepare/fix/fixatives/formalin-buffered.htm
  2. The Neutral Buffered Formalin has to be injected into the specimen for animals with hard shells such as crabs, or have thick flesh and body cavity such as fishes, amphibians and reptiles.  The specimens have to be incubated in the fixative solution for at least three days until the specimens are well fixed.
  3. After the specimens have hardened, they are rinsed with water to remove the Neutral Buffered Formalin untill the smell is not too strong.
  4. Specimens are placed in an air tight container (glass bottles, plastic containers or vials) that are filled with 70% alcohol (Figure 8).
  5. For large sized specimens (>50 cm), when the specimens are handed in, they should be wrapped in gauze that had been soaked in 70% alcohol and placed inside the container.

Figure 8. Example of a specimen that had been fixated with Neutral Buffered Formalin and soaked in 70% alcohol (Source of the picture:  Royal BC Museum).

Intact specimens preserved in a freezer and handed in using an icebox.

Examples of specimens that could be handed in using this method are freshwater fishes, ocean fishes, shrimps, squids and jelly fishes (Figure 9).

Figure 9. Example of specimens preserved in an icebox  (Source of the picture:  http://www.coolersonsale.com).

  1. Plant/animal specimens are not advised to be entrusted to someone else.

    Directly bring the specimens with the proof of payment and the request for the identification or determination letter, the original as well as a copy, to:

Herbarium Bandungense and Zoology Museum Building, SITH – ITB

ITB Jatinangor Campus, Labtek V C, next to the Al-Jabbar Mosque.

Contact person for Plant Determination:

Arifin Surya Dwipa Irsyam B.Sc, M.Sc. (0857 8052 6704)

Contact person for Animal Determination:

Ganjar Cahyadi S.Si (WA: 0857 2033 9120)

The letter of the results of the determination can be picked up at the Administration Office   SITH ITB, Labtek XI Building, 1st floor, SITH ITB.

 

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